Induced Ovulation of Southern Flounder Paralichthys lethostigma Using Gonadotropin Releasing Hormone Analogue Implants

Journal of the World Aquaculture Society - Tập 27 Số 2 - Trang 143-152 - 1996
David L. Berlinsky1,2, William King1, Theodore I. J. Smith3, Richard D. Hamilton4, J. Douglas Holloway4, Craig V. Sullivan1
1Department of Zoology, North Carolina State University, Campus Box 7617, Raleigh. North Carolina 27695 USA
2University of Rhode Island, Department of Fisheries, Animal and Veterinary Science, East Farm Building 14, Rte. 108, Kingston, Rhode Is land 02881 USA.
3South Carolina Department of Natural Resources, Marine Resources Research Institute, P.O. Box 12559, Charleston, South Carolina 29422 USA
4South Carolina Department of Natural Resources, Waddell Mariculture Center, P.O. Box 809, Bluffton. South Carolina 29910 USA

Tóm tắt

Abstract

Implanted pellets that provide a sustained release of [D‐Ala6 Des‐Gly10] LHRH‐ethylamide (GnRHa) were used to induce maturation and ovulation of Southern flounder Paralichthys lethostigma. Of the 12 females whose ovaries contained follicles with a maximum diameter ≥500 μm, 11 ovulated for the first time within 90 h of hormone implantation. Only 1 fish with a maximum follicle diameter less than 500 μm ovulated within 2 wk after implantation. Ovulated eggs were manually stripped from the females and mixed with sperm from several males. Most females were spawned 1 to 3 times on consecutive days with variable fertility. One female was spawned 11 times producing 668,000 eggs. Fertility was evaluated by examining the incubated eggs for early stages of embryonic cleavage. The percentage of fertile eggs in subsamples of incubated eggs ranged from 7–95%. The results indicate that GnRHa implants can be used to induce repeated ovulation in this species. The variability in fertility is discussed in relation to egg quality.

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Journal of the World Aquaculture Society

Tập 27 Số 2

143-152

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