Increased PELP1 expression in rat periodontal ligament tissue in response to estrogens treatment
Tóm tắt
Estrogens and their receptors are important factors involved in periodontal ligament (PDL) tissue health. As a regulator of estrogen receptors (ER), the proline-, glutamic acid-, and leucine-rich protein 1 (PELP1) may play a role in alveolar bone formation and PDL homeostasis. The aim of the present study was to observe PELP1 expression in rat PDL tissue during estrogen levels manipulations. Twenty-one 8-week old normal female Sprague–Dawley rats were randomly divided into three equal groups: sham-operated controls, ovariectomized (OVX) group, and OVX given 17β-estradiol intraperitoneally (OVX + E2) for 16 weeks. PELP1 expression was down-regulated in the OVX group and was up-regulated in the OVX + E2 group. Periodontal ligament fibroblast cells (PDLFCs) were isolated from PDL tissue, and characterized by immunohistochemical staining. Estradiol treatment of PDLFCs induced PELP1 protein level compared to untreated cells. PELP1 mRNA expression in estradiol-treated cells was relatively low at the beginning of treatment and then steadily increased from hour 4. In conclusion, results indicate that PELP1 is expressed in rat PDL tissue and PDLFCs, and that its expression is up-regulated during estrogen treatment.
Tài liệu tham khảo
Abdul-Majeed S et al (2012) Effects of tocotrienol and lovastatin combination on osteoblast and osteoclast activity in estrogen-deficient osteoporosis. Evid Based Complement Alternat Med 2012:960742. doi:10.1155/2012/960742
Abujazia MA et al (2012) The effects of virgin coconut oil on bone oxidative status in ovariectomised rat. Evid Based Complement Alternat Med 2012:525079. doi:10.1155/2012/525079
Alegria-Schaffer A et al (2009) Performing and optimizing Western blots with an emphasis on chemiluminescent detection. Methods Enzymol 463:573–599. doi:10.1016/S0076-6879(09)63033-0
Balasenthil S, Vadlamudi RK (2003) Functional interactions between the estrogen receptor coactivator PELP1/MNAR and retinoblastoma protein. J Biol Chem 278(24):22119–22127. doi:10.1074/jbc.M212822200M212822200
Barletta F et al (2004) Characterization of the interactions of estrogen receptor and MNAR in the activation of cSrc. Mol Endocrinol 18(5):1096–1108. doi:10.1210/me.2003-0335me.2003-0335
Bord S et al (2003) The effects of estrogen on osteoprotegerin, RANKL, and estrogen receptor expression in human osteoblasts. Bone 32(2):136–141. doi:10.1016/S8756-3282(02)00953-5
Brann DW et al (2008) PELP1–a novel estrogen receptor-interacting protein. Mol Cell Endocrinol 290(1–2):2–7. doi:10.1016/j.mce.2008.04.019
Cao M et al (2007) The expression of estrogen receptors and the effects of estrogen on human periodontal ligament cells. Methods Find Exp Clin Pharmacol 29(5):329–335. doi:10.1358/mf.2007.29.5.1117560
Cheskis BJ et al (2008) MNAR plays an important role in ERa activation of Src/MAPK and PI3 K/Akt signaling pathways. Steroids 73(9–10):901–905. doi:10.1016/j.steroids.2007.12.028
Chimutengwende-Gordon M, Khan WS (2011) Advances in the use of stem cells and tissue engineering applications in bone repair. Curr Stem Cell Res Ther 7(2):122–126. doi:BSP/CSCRT/E-Pub/00115
DeFriend DJ et al (1994) Investigation of a new pure antiestrogen (ICI 182780) in women with primary breast cancer. Cancer Res 54(2):408–414
Dobrzycka KM et al (2003) Estrogen receptor corepressors—a role in human breast cancer? Endocr Relat Cancer 10(4):517–536
Du J et al (2012) The expression pattern of FHL2 during mouse molar development. J Mol Histol 43(3):289–295. doi:10.1007/s10735-012-9409-z
Greger JG et al (2006) Characterization of MNAR expression. Steroids 71(4):317–322. doi:10.1016/j.steroids.2005.09.016
Grivas PD et al (2009) Expression of ERalpha, ERbeta and co-regulator PELP1/MNAR in colorectal cancer: prognostic significance and clinicopathologic correlations. Cell Oncol 31(3):235–247. doi:10.3233/CLO-2009-0467
Hongo H et al (2011) Frontiers in vitamin D; basic research and clinical application. A review on histological findings in bones administered with eldecalcitol. Clin Calcium 21(11):63–70. doi:CliCa111116471654
Ibi M et al (2007) Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments. Arch Oral Biol 52(10):1002–1008. doi:10.1016/j.archoralbio.2007.04.009
Isaka J et al (2001) Participation of periodontal ligament cells with regeneration of alveolar bone. J Periodontol 72(3):314–323. doi:10.1902/jop
Jonsson D et al (2011) The human periodontal ligament cell: a fibroblast-like cell acting as an immune cell. J Periodontal Res 46(2):153–157. doi:10.1111/j.1600-0765.2010.01331.x
Kawase T et al (1988) Alkaline phosphatase of human periodontal ligament fibroblast-like cells. Adv Dent Res 2(2):234–239
Liang L et al (2008) Effect of estrogen receptor beta on the osteoblastic differentiation function of human periodontal ligament cells. Arch Oral Biol 53(6):553–557. doi:10.1016/j.archoralbio.2007.12.011
Liu Y et al (2008) Periodontal ligament stem cell-mediated treatment for periodontitis in miniature swine. Stem Cells 26(4):1065–1073. doi:10.1634/stemcells.2007-0734
Machtei EE et al (2004) The effect of menstrual cycle on periodontal health. J Periodontol 75(3):408–412. doi:10.1902
Meyer MR et al (2009) Non-genomic regulation of vascular cell function and growth by estrogen. Mol Cell Endocrinol 308(1–2):9–16. doi:10.1016/j.mce.2009.03.009
Mishra SK et al (2004) Cloning and functional characterization of PELP1/MNAR promoter. Gene 330:115–122. doi:10.1016/j.gene.2004.01.011S037811190400037X
Nair SS et al (2004) Potential role of a novel transcriptional coactivator PELP1 in histone H1 displacement in cancer cells. Cancer Res 64(18):6416–6423. doi:10.1158/0008-5472.CAN-04-178664/18/6416
Orwoll ES et al (2009) Sex steroids, periodontal health, and tooth loss in older men. J Dent Res 88(8):704–708. doi:10.1177/0022034509341013
Pawlak J, Beyer C (2005) Developmental expression of MNAR mRNA in the mouse brain. Cell Tissue Res 320(3):545–549. doi:10.1007/s00441-005-1090-z
Prisby RD et al (2012) Aging and estrogen status: a possible endothelium-dependent vascular coupling mechanism in bone remodeling. PLoS ONE 7(11):e48564. doi:10.1371/journal.pone.0048564PONE-D-12-23015
Rachon D et al (2008) Effects of black cohosh extract on body weight gain, intra-abdominal fat accumulation, plasma lipids and glucose tolerance in ovariectomized Sprague-Dawley rats. Maturitas 60(3–4):209–215. doi:10.1016/j.maturitas.2008.06.001
Seo BM et al (2004) Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet 364(9429):149–155. doi:10.1016/S0140-6736(04)16627-0S0140673604166270
Sims NA et al (1996) Estradiol treatment transiently increases trabecular bone volume in ovariectomized rats. Bone 19(5):455–461. doi:S8756328296002426
Smuc T et al (2009) Disturbed estrogen and progesterone action in ovarian endometriosis. Mol Cell Endocrinol 301(1–2):59–64. doi:10.1016/j.mce.2008.07.020
Taichman LS, Eklund SA (2005) Oral contraceptives and periodontal diseases: rethinking the association based upon analysis of National Health and Nutrition Examination Survey data. J Periodontol 76(8):1374–1385. doi:10.1902
Techawattanawisal W et al (2007) Isolation of multipotent stem cells from adult rat periodontal ligament by neurosphere-forming culture system. Biochem Biophys Res Commun 357(4):917–923. doi:10.1016/j.bbrc.2007.04.031
Tilakaratne A et al (2000) Effects of hormonal contraceptives on the periodontium, in a population of rural Sri-Lankan women. J Clin Periodontol 27(10):753–757
Toran-Allerand CD (2004) Estrogen and the brain: beyond ER-alpha and ER-beta. Exp Gerontol 39(11–12):1579–1586. doi:10.1016/j.exger.2004.05.006
Vadlamudi RK, Kumar R (2007) Functional and biological properties of the nuclear receptor coregulator PELP1/MNAR. Nucl Recept Signal 5:e004. doi:10.1621/nrs.05004
Vadlamudi RK, Wang RA, Mazumdar A et al (2001) Molecular cloning and characterization of PELP1, a novel human coregulator of estrogen receptor alpha. J Biol Chem 276(41):38272–38279. doi:10.1074/jbc.M103783200M103783200
Vadlamudi RK et al (2004) Deregulation of estrogen receptor coactivator proline-, glutamic acid-, and leucine-rich protein-1/modulator of nongenomic activity of estrogen receptor in human endometrial tumors. J Clin Endocrinol Metab 89(12):6130–6138. doi:10.1210/jc.2004-0909
Vadlamudi RK et al (2005) Novel estrogen receptor coactivator PELP1/MNAR gene and ERbeta expression in salivary duct adenocarcinoma: potential therapeutic targets. Hum Pathol 36(6):670–675. doi:10.1016/j.humpath.2005.03.016
Wang Q et al (2006) Temporal expression of estrogen receptor alpha in rat bone marrow mesenchymal stem cells. Biochem Biophys Res Commun 347(1):117–123. doi:10.1016/j.bbrc.2006.06.070
Williams MD et al (2007) Differential expression of hormonal and growth factor receptors in salivary duct carcinomas: biologic significance and potential role in therapeutic stratification of patients. Am J Surg Pathol 31(11):1645–1652. doi:10.1097/PAS.0b013e3180caa09900000478-200711000-00003
Wong CW et al (2002) Estrogen receptor-interacting protein that modulates its nongenomic activity-crosstalk with Src/Erk phosphorylation cascade. Proc Natl Acad Sci USA 99(23):14783–14788. doi:10.1073/pnas.192569699192569699
Yamaji Y et al (1995) Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides. Infect Immun 63(9):3576–3581
Zilli M et al (2009) Molecular mechanisms of endocrine resistance and their implication in the therapy of breast cancer. Biochim Biophys Acta 1795(1):62–81. doi:10.1016/j.bbcan.2008.08.003