Incidence of Antibiotic Resistance inCampylobacter jejuniIsolated in Alberta, Canada, from 1999 to 2002, with Special Reference totet(O)-Mediated Tetracycline Resistance

Antimicrobial Agents and Chemotherapy - Tập 48 Số 9 - Trang 3442-3450 - 2004
Amera Gibreel1, Dobryan M. Tracz1, Lisa Nonaka1, Trinh M. Ngo1, Sean R. Connell1, Diane E. Taylor2,1
1Departments of Medical Microbiology and Immunology
2Biological Sciences, University of Alberta, Edmonton, Alberta, Canada

Tóm tắt

ABSTRACTOf 203 human clinical isolates ofCampylobacter jejunifrom Alberta, Canada (1999 to 2002), 101 isolates (50%) were resistant to at least 64 μg of tetracycline/ml, with four isolates exhibiting higher levels of tetracycline resistance (512 μg/ml). In total, the MICs for 37% of tetracycline-resistant isolates (256 to 512 μg/ml) were higher than those previously reported inC. jejuni(64 to 128 μg/ml). In the tetracycline-resistant clinical isolates, 67% contained plasmids and all contained thetet(O) gene. Four isolates resistant to high levels of tetracycline (MIC = 512 μg/ml) contained plasmids carrying thetet(O) gene, which could be transferred to other isolates ofC. jejuni. The tetracycline MICs for transconjugants were comparable to those of the donors. Cloning oftet(O) from the four high-level tetracycline-resistant isolates conferred an MIC of 32 μg/ml forEscherichia coliDH5α. In contrast, transfer to a strain ofC. jejuniby using mobilization conferred an MIC of 128 μg/ml. DNA sequence analysis determined that thetet(O) genes encoding lower MICs (64 to 128 μg/ml) were identical to one other, although thetet(O) genes encoding a 512-μg/ml MIC demonstrated several nucleotide substitutions. The quinolone resistance determining region of four ciprofloxacin-resistant isolates (2%) was analyzed, and resistance was associated with a chromosomal mutation in thegyrAgene resulting in a Thr-86-Ile substitution. In addition, six kanamycin-resistant isolates contained large plasmids that carry theaphA-3marker coding for 3′-aminoglycoside phosphotransferase. Resistance to erythromycin was not detected in 203 isolates. In general, resistance to most antibiotics inC. jejuniremains low, except for resistance to tetracycline, which has increased from about 8 to 50% over the past 20 years.

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Antimicrobial Agents and Chemotherapy

Tập 48 Số 9

3442-3450

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