In vitro antioxidant analysis of supercritical fluid extracts from rosemary (Rosmarinus officinalis L.)
Tóm tắt
Different supercritical fluid extraction conditions were tested on rosemary leaves using a pilot-plant-scale extractor. Each of them provided two separated fractions which were characterized chemically by liquid chromatography–diode-array detection–mass spectrometry using electrospray in the positive mode. Twelve compounds were identified, including phenolic diterpenes and flavonoids. Owing to the lack of available standards, only carnosic acid could be quantified. The antioxidant activity of the extracts was determined by the 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) test and the β-carotene bleaching test was used to study the differences in behavior of antioxidants in an emulsified medium for some of the extracts obtained. The results of the correlation studies to examine the relationship between the antioxidant activity (EC50), obtained by using the DPPH test, and the concentration of all the compounds detected in the extracts showed that carnosic acid was the more correlated compound, with a coefficient of correlation of −0.87. Using forward stepwise multiple linear regression, carnosic acid, methyl carnosate and carnosol were the compounds selected to predict the mentioned activity, with a value of 0.95 for the coefficient of determination.
Tài liệu tham khảo
Chipault JR, Mizumo GR, Hawkins JM, Lundberg WO (1952) Food Res 17:46
Halliwell B (1999) Food Sci Agric Chem 67–109
Halliwell B, Aesbach R, Löliger J, Aruoma OI (1995) Food Chem Toxicol 33:601–607
Madhavi DL, Despande SS, Salunkhe DK (1996) Food antioxidants, Marcel Dekker, New York
Inatani R, Nakatani N, Fuwa H (1983) Agric Biol Chem 47:521–528
Schwarz K, Ternes W (1992) Z. Lebensm Unters Forsch 195:99–103
Schwarz K, Ternes W, Schmauderer E (1992) Z Lebensm Unters Forsch. 195:104–107
Cuvelier ME, Berset C, Richard H (1994) J Agric Food Chem 42:665–669
Aruoma OI, Halliwell B, Aeschbach R, Loligers J (1992) Xenobiotica 22:257–268
Frankel EN, Huang S-W, Aeschbach R, Prior E (1996) J Agric Food Chem 44:131–135
Frankel EN, Huang S-W, Prior E, Aeschbach R (1996) J Sci Food Agric 72:201–208
Tena MT, Valcárcel M, Hidalgo P, Ubera JL (1997) Anal Chem 69:521–526
Reverchon E, Donsi G, Pota F (1992) Ital J Food Sci 3:187–194
Ibañez E, Oca A, Murga G de Lopez-Sebastian S, Tabera J, Reglero G (1999) J Agric Food Chem 47:1400–1404
Cuvelier ME, Richard H, Berset C (1996) J Am Oil Chem Soc 73:645–652
Señoráns FJ, Ibañez E, Cavero S, Tabera J, Reglero G (2000) J Chromatogr A 870:491–499
Brand-Williams W, Cuvelier ME, Berset C (1995) Lebens. Wiss Technol 28:25–30
Velioglu YS, Mazza G, Gao L, Oomah BD (1998) J Agric Food Chem 46:4113–4117
Tsuda T, Mizuno K, Ohshima K, Kawakishi S, Osawa S (1995) J Agric Food Chem 43:2803–2806
Richheimer SL, Bernart MW, King GA, Kent MC, Bailey DT (1996) J Am Oil Chem Soc 73:507–514
Luis JG, Quiñones W, Grillo TA, Kishi MP (1994) Phytochem. 35:1373–1374
Munné-Bosch S, Alegre L (2001) Plant Phys 125:1094–1102
Masuda T, Inaba Y, Maekawa T, Takeda Y, Tamura H, Yamaguchi H (2002) J Agric Food Chem 50:5863–5869