In vitro, acute and subchronic evaluation of the antidiabetic activity of Atractylis flava Desf n-butanol extract in alloxan-diabetic rats
Tóm tắt
Diabetes mellitus is a serious complex multifactorial disorder that imposes huge health and economic burden on societies. Because the currently available medications have many drawbacks, it's important to look for alternative therapies. Medicinal plants utilized in folk medicine are ideal candidates. Therefore, this work assessed the antidiabetic action of n-butanol extract from the whole plant Atractylis flava Desf (BEAF). These ethnomedicinal properties of BEAF were scientifically validated using in vitro and in vivo assays. In vitro antidiabetic effect of the BEAF was conducted using α-Glucosidase and α-Amylase assays. While the antihyperglycemic activity was assessed using two rat models: Alloxan-induced diabetic rats and oral glucose challenged rats. Experimental diabetes was induced by a single intraperitoneal injection of alloxan at a dose of 150 mg/kg and animals with fasting blood glucose levels (BGL) > 200 mg/dL were considered diabetic. Glibenclamide (5 mg/kg) was used as a typical drug. The BEAF at all tested dose levels (100, 250, and 500 mg/kg) showed a significant decrease in blood glucose level in all the two animal models. Besides, the plant extract exhibited a potent inhibitory effect on α-Amylase and α-Glucosidase activity at a concentration of 1000 µg/mL with 76.17% and 89.37%, respectively. BEAF exerts in vitro and in vivo antidiabetic effects, these results suggest that the plant extract can be a therapeutic resource in the treatment of diabetes and hyperlipidemia.
Tài liệu tham khảo
Wu YL, Ding YP, Gao J, Tanaka Y, Zhang W (2013) Risk factors and primary prevention trials for type 1 diabetes. Int J Biol Sci 9(7):666–679
Hu FB (2011) Globalization of diabetes: the role of diet, lifestyle, and genes. Diabetes Care 34(6):1249–1257
Khan V, Najmi AK, Akhtar M, Aqil M, Mujeeb M, Pillai KK (2012) A pharmacological appraisal of medicinal plants with antidiabetic potential. J Pharm Bioallied Sci 4:27–42
Baldea LAN, Martineau LC, Benhaddou-Andaloussi A, Arnason JT, Levy E, Haddad PS (2012) Inhibition of intestinal glucose absorption by anti-diabetic medicinal plants derived from the James Bay Cree traditional pharmacopeia. J Ethnopharmacol 132(2):473–482
Quezel P, Santa S (1963) Nouvelle flore de l’Algérie et des régions désertiques méridionales. CNRS, Paris, France (1–2)
Akram MM, Naima B, Eddine MS, Soumia B (2018) Anti-Inflammatory, antipyretic and acute toxicity effects of n-butanol extract of Atractylis Flava desf in rats. Pharmacog J 10(4):763–767
Akram MM, Naima B, Eddine MS, Joubert O, Doumandji ZM, Safar R (2019) Gene expression levels of selected factors in monocytic leukemia cell line THP-1 upon treatment with n-butanol extract of Atractylis flava Desf against Cancer J Young Pharm. 11(1):36–9.
Melakhessou MA, Benkiki N, Marref SE (2018) Determination of antioxidant capacity, flavonoids and total phenolic content of extracts from Atractylis flava Desf. Res J Pharm Tech 11(12):5221–5226. https://doi.org/10.5958/0974-360X.2018.00952.6
Daniele C, Dahamna S, Firuzi O, Sekfali N, Saso L, Mazzanti G (2005) Atractylis gummifera L Poisoning: an ethnopharmacological review. J Ethnopharmacol 97(2):175–181
El Rhaffari L, Zaid A (2002) Pratique de la phytothérapie dans le sud-est du Maroc (Tafilalet). Un savoir empirique pour une pharmacopée rénoveé. Dans J. Fleurentin (éd.), Des sources du savoir auxmédicaments du futur, IRD editions, Paris. 293–318
Chabani S, Haba H, Lavaud C, Benkhaled M, Harakat D (2013) Flavonoid glycosides and triterpenoids from Atractylis flava. Phytochem Lett 6(1):9–13
Islam MN, Ishita IJ, Jung HA (2014) Choi J S (2014) Vicenin 2 isolated from Artemisia capillaris exhibited potent anti-glycation properties. Food Chem Toxicol 69:55–62
Goto T, Horita M, Nagai H, Nagatomo A, Nishida N, Matsuura Y, Nagaoka S (2012) Tiliroside a glycosidic flavonoid, inhibits carbohydrate digestion and glucose absorption in the gastrointestinal tract. Mol Nutr Food Res 56(3):435–445
Mccue P, Shetty K (2004) Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asian Pac J Clin Nutr 2004(13):101–106
Kim YM, Jeong YK, Wang MH, Lee WY, Rhee HI (2005) Inhibitory effects of pine bark extract on alpha glucosidase activity and postprandial hyperglycemia. Nutrition 21:756–761
Babu V, Ganga DT, Subramonium A (2002) Antihyperglycaemic activity of Cassia kleinii leaf extract in glucose fed normal ratsand alloxan induced diabetic rats. Indian J Pharmacol 34:409–413
Diatewa M, Badila Samba C, Hondi Assah TC, Abena AA (2004) Hypoglycemic and antihyperglycemiceffects of diethyl ether fraction isolated from the aqueous extract of the leaves of Cogniauxia podoleana Baillon in normal and alloxan-induced diabetic rats. J Ethnopharmacol 92:229–232
Usman UZ, Bakar ABA, Mohamed M (2018) Propolis improves pregnancy outcomes and placental oxidative stress status in streptozotocin-induced diabetic rats. BMC Complement Altern Med 18:324. https://doi.org/10.1186/s12906-018-2391-6
Nair SS, Kavrekar V, Mishra A (2013) In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. Eur J Exp Biol 3:128–132
Figueiredo-González M, Reboredo-Rodríguez P, González-Barreiro C, Simal-Gándara J, Valentão P, Carrasco-Pancorbo A, Andrade PB, Cancho-Grande B (2018) Evaluation of the neuroprotective and antidiabetic potential of phenol-rich extracts from virgin olive oils by in vitro assays. Food Res Int 106:558–567
Etuk EU (2010) (2010) Animals models for studying diabetes mellitus. Agric Biol J N Am 1(2):130–134
Tesfaye A, Makonnen E, Gedamu S (2016) Hypoglycemic and antihyperglycemicactivity of aqueous extract of Justicia Schimperiana leavesin normal and streptozotocin-induced diabetic mice. IJPSR 7(2):110–113
Yadav S, Vats V, Dhunnoo Y (2002) Grover JK (2002) Hypoglycemic and antihyperglycemic activity of Murraya koenigii leaves in diabetic rats. J Ethnopharmacol 82:111–116
Dabla PK (2010) Renal function in diabetic nephropathy World. J Diabetes 1:48–56
Carmena R (2005) Type 2 diabetes, dyslipidemia, and vascular risk: Rationale and evidence for correcting the lipid imbalance. Am Heart J 150:859–870
Carmena R, Betteridge DJ (2004) Statins and diabetes. Semin Vasc Med 4:321–332
Solano MP (2006) Goldberg RB (2006) Lipid management in type 2 diabetes. Clin Diabetes 24:27–32
Papanas N, Maltezos E (2009) Oral antidiabetic agents: Antiatherosclerotic properties beyond glucose lowering? Curr Pharm Des 15:3179–3192
Molavi B, Rassouli N, Bagwe S, Rasouli N (2007) A review of thiazolidinediones and metformin in the treatment oftype 2 diabetes with focus on cardiovascular complications. Vasc Health Risk Manag 3:967–973