In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles

American Association for the Advancement of Science (AAAS) - Tập 298 Số 5599 - Trang 1759-1762 - 2002
Benoît Dubertret1,2, Paris A. Skourides3, David J. Norris4,2, Vincent Noireaux5,6, Ali H. Brivanlou3, Albert Libchaber5,2
1Center for Studies in Physics and Biology, Laboratory of Molecular Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. [email protected]
2NEC Research Institute, 4 Independence Way, Princeton, NJ 08540 USA
3Laboratory of Molecular Embryology, The Rockefeller University, 1230 York Avenue, New York, NY 10021 USA
4Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455, USA
5Center for Studies in Physics and Biology,
6Rockefeller University

Tóm tắt

Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this problem, we encapsulated individual nanocrystals in phospholipid block–copolymer micelles and demonstrated both in vitro and in vivo imaging. When conjugated to DNA, the nanocrystal-micelles acted as in vitro fluorescent probes to hybridize to specific complementary sequences. Moreover, when injected into Xenopus embryos, the nanocrystal-micelles were stable, nontoxic (<5 × 10 9 nanocrystals per cell), cell autonomous, and slow to photobleach. Nanocrystal fluorescence could be followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis.

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We thank H. Shio for assistance with electron microscopy and R. Harland (University of California Berkeley) for membrane-GFP constructs. B.D. was supported in part by the Norman and Rosita Winston Fellowship. V.N. was supported in part by the Burroughs Wellcome Foundation. P.S. and A.H.B. are supported by funds from the Steinbach Fund and the Rockefeller University. B.D. acknowledges discussions with S. M. Simon and J. K. Jaiswal.

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American Association for the Advancement of Science (AAAS)

Tập 298 Số 5599

1759-1762

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