In Vitro Propagation of Two Perkinsus spp. Parasites from Japanese Manila Clams Venerupis philippinarum and Description of Perkinsus honshuensis n. sp.

Journal of Eukaryotic Microbiology - Tập 53 Số 5 - Trang 316-326 - 2006
Christopher F. Dungan1, Kimberly S. Reece
1Maryland Department of Natural Resources, Cooperative Oxford Laboratory, 904 S. Morris St., Oxford, Maryland 21654, USA. [email protected]

Tóm tắt

ABSTRACT. Perkinsus species are destructive parasites of commercial Manila clams, Venerupis philippinarum, in Japan, Korea, and Spain. However, in vitro parasite cultures from this important host clam are not available. Tissues of Manila clams collected during April 2002 in Gokasho Bay, Japan harbored Perkinsus sp. parasites at a 97% prevalence (28/29) of moderate‐ and high‐intensity infections. Perkinsus sp. cells in tissue samples were enlarged in alternative Ray's fluid thioglycollate medium, before propagation in DME:Ham's F‐12 Perkinsus sp. culture medium. Enlarged parasite hypnospores zoosporulated at high frequencies to release motile zoospores, which gave rise to continuous schizogonic cell lines that also zoosporulated continuously at low frequencies. Four Perkinsus sp. in vitro isolates comprising two distinct morphotypes were cryopreserved, cloned, and archived for public distribution. For three isolates of one morphotype, nucleotide sequences of the ribosomal DNA internal transcribed spacer region, of the large subunit rRNA gene, and of actin genes, were consistent with those reported for P. olseni. Similar sequences from one morphologically unique isolate differed from those of all described Perkinsus species. These results show that at least two Perkinsus spp. infect Japanese Manila clams, and that one represents a new species, Perkinsus honshuensis n. sp.

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Tài liệu tham khảo

10.1111/j.1550-7408.2005.00053.x

10.3354/dao048043

Blackbourn J., 1998, Perkinsus qugwadi sp. nov. (incertae sedis), a pathogenic protozoan parasite of Japanese scallops, Patinopecten yessoensis, cultured in British Colombia, Canada, Can. J. Zool., 76, 942, 10.1139/z98-015

10.1111/j.1550-7408.2005.05-00035.x

10.1111/j.1550-7408.2002.tb00333.x

10.3354/dao052217

Choi K.‐S., 1997, Report on the occurrence of Perkinsus sp. In the Manila clam, Ruditapes philippinarum in Korea, J. Aquaculture, 10, 227

Choi K. S., 1989, The energetic cost of Perkinsus marinus parasitism in oysters, quantification of the fluid thioglycollate method, 8, 125

10.1111/j.1550-7408.1995.tb01598.x

10.3354/dao050067

10.3354/dao015009

Elston R. A., 2004, Perkinsus sp. Infection risk for Manila clams, Venerupis philippinarum (A. Adam and Reeve, 1850), on the Pacific coast of North and Central America, J. Shellfish Res., 23, 101

10.1016/0166-6851(94)90126-0

10.3147/jsfp.33.473

Hine P. M., 2001, Proceedings of the OIE International Conference on Risk Analysis in Aquatic Animal Health, 71

Hine P. M., 2002, The distribution of Perkinsus olseni in New Zealand bivalve molluscs, Surveillance, 29, 8

10.1577/1548-8659(1995)124<0144:IMFTIO>2.3.CO;2

Le Borgne Y., 1996, Echanges internationaux des mollusques bivalves et situation actuelle en France et en Europe, Rev. Sci. Tech. Off. Int. Epiz., 15, 491, 10.20506/rst.15.2.933

10.1016/0022-2011(81)90073-2

10.1016/B978-0-12-550755-4.50024-0

Mackin J. G., 1961, Oyster disease caused by Dermocystidium marinum and other microorganisms in Louisiana, Inst. Mar. Sci. Publ., 7, 133

10.3147/jsfp.34.127

10.1007/BF00318165

Murrell A., 2002, Synonymy of Perkinsus olseni (Lester & Davis 1981) and Perkinsus atlanticus (Azevedo 1989), and an update on the phylogenetic position of the genus Perkinsus, Bull. Eur. Ass. Fish Pathol., 22, 258

Nickens A. D., 2002, An improved procedure to count Perkinsus marinus in eastern oyster hemolymph, J. Shellfish Res., 21, 725

10.1016/S0044-8486(01)00619-6

Park K.‐I., 2002, Development of a PCR assay for detection of the protozoan parasite Perkinsus, Korean J. Environ. Biol., 20, 109

10.3354/dao066255

Ray S. M., 1963, A review of the culture method for detecting Dermocystidium marinum, with suggested modifications and precautions, Proc. Natl. Shellfish. Assoc., 54, 55

10.1645/0022-3395(2000)086[0972:COTRRL]2.0.CO;2

10.1016/0044-8486(94)00393-3

10.1016/0044-8486(94)00391-Z

Sagristá E., 1996, Ultrastructural data on the life cycle of the parasite, Perkinsus atlanticus (Apicomplexa), on the clam, Ruditapes philippinarum, in the Mediterranean, Sci. Mar., 60, 283

Swofford D. L., 2002, PAUP* 4.0: Phylogenetic Analysis Using Parsimony (and Other Methods)

10.1093/nar/22.22.4673

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Journal of Eukaryotic Microbiology

Tập 53 Số 5

316-326

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