Improving the quality of Vernonia amygdalina extract by ultrasound‐assisted extraction coupled with gas bubble flotation

Journal of Food Process Engineering - Tập 42 Số 8 - 2019
Trong Quan Luu1, Phat T. Le1, Khoa C. M. Le1, An H. T. Phan1, Kim Zitzmann2, Kim Thien Phuc Nguyen1, Nam Van Ho Phan3, Khoi Tan Nguyen1,4
1School of Biotechnology, International University, Viet Nam National University, Ho Chi Minh City, Vietnam
2College of Science, University College Dublin, Belfield, Dublin, Ireland
3Ho Chi Minh City Medicine and Pharmacy University, Ho Chi Minh City, Vietnam
4School of Chemical Engineering, The University of Queensland, Brisbane, Queensland, Australia

Tóm tắt

Abstract

Vernonia amygdalina is an herb commonly used in folk medicine; It possesses various compounds with therapeutic capabilities. Traditional extraction approaches applied to this plant may destroy its bioactive compounds and inherently contaminate them with toxic organic solvents. In this study, ultrasound‐assisted extraction combined with gas‐bubble flotation was applied to increase V. amygdalina extraction yield. The efficiency of water maceration was increased by 200%, without the drawbacks of the conventional extraction methods. The total phenolic content yield was 189 ± 12.7 (mg GAE/g dry weight) with a condensation efficiency of 115 ± 5.7%; and the total flavonoid content was 6.44 ± 1.68 (mg QE/g dry weight). Additionally, the antioxidant activity of the condensed foam was enhanced up to 120%. Analysis of the extract by FTIR revealed the presence of extractable chemical families: sesquiterpene lactones, saponins and flavonoids, and that the chemical characteristics of the extract were also preserved due to mild extracting conditions. Overall, this method is effective for extracting bioactive compounds from herbs with appealing advantages: no thermal degradation, organic solvent free extraction, and low energy consumption.

Practical Applications

The utilization of ultrasound‐assisted extraction and gas bubble flotation has been proven to be effective in condensing the aqueous extract of Vernonia amygdalina. Gas bubble flotation was shown to condense the herb extract by 120%, which is considered efficient given the simplicity and energy consumption of the process.

Từ khóa


Tài liệu tham khảo

10.1186/s12936-015-0812-2

10.1016/j.vibspec.2017.05.008

10.1038/nprot.2007.102

10.1007/s11694-017-9642-y

10.1016/j.addr.2007.05.007

10.1371/journal.pone.0148758

10.2478/s11532-013-0395-0

10.1016/j.jfoodeng.2013.01.014

Azwanida N., 2015, A review on the extraction methods use in medicinal plants, principle, strength and limitation, Medicinal and Aromatic Plants, 4, 196

10.1021/jf020917c

10.1021/np049743e

10.1016/j.cis.2017.02.008

10.1021/jf001413m

10.1016/j.ultsonch.2016.06.035

10.1016/j.ultsonch.2008.07.007

10.1038/srep42717

10.1021/ci034243x

10.2478/s11532-009-0125-9

10.1007/s11483-015-9420-5

Eberhardt T. L., 2007, Chinese tallow tree (Sapium sebiferum) utilization: Characterization of extractives and cell‐wall chemistry, Wood and Fiber Science, 39, 319

10.1016/j.jep.2005.12.016

10.1080/13880200701213070

10.1111/jfpe.12358

10.1016/j.phytol.2012.06.012

10.1016/j.crci.2016.01.018

10.3390/foods7100164

10.1016/S0924-2031(00)00091-6

10.1021/jf9001439

10.1016/j.ultsonch.2013.01.020

10.1080/10408390600698197

Hazra K. M., 2007, Isolation of antibacterial pentahydroxy flavones from the seeds of Mimusops elengi Linn, African Journal of Biotechnology, 6, 1446

10.1016/j.drudis.2010.05.016

10.1016/j.ultsonch.2011.11.001

Hostettmann K., 2001, Modern screening techniques for plant extracts, Pharmaceutical Biology, 39, 18, 10.1076/phbi.39.7.18.5867

10.1016/S1350-4177(01)00093-1

10.1021/jf00047a015

10.1271/bbb.57.833

10.1111/1541-4337.12005

Kalita Pallab B. T. K., 2013, Estimation of Total flavonoids content (TFC) and anti oxidant activities of Methanolic whole plant extraction of Biophytum Sensitivum Lin, Journal of Drug Delivery & Therapeutics, 3, 33

10.1016/j.foodchem.2008.08.041

10.1016/j.foodchem.2015.08.068

10.1016/j.ultsonch.2004.12.003

10.1111/j.1745-4530.2010.00601.x

10.1016/j.foodchem.2009.05.075

10.1111/jfpe.12322

10.1016/j.tet.2016.12.014

10.1002/jctb.612

10.3390/agronomy7030047

10.1016/S0378-8741(99)00189-0

10.1016/j.bjbas.2017.07.007

Ozen C. C. a. B., 2015, Monitoring of wine process and prediction of its parameters with mid‐infrared spectroscopy, Journal of Food Process Engineering, 40, 1

10.1007/s12161-014-9814-x

10.1016/j.jfoodeng.2012.01.026

10.1016/S0021-9673(03)01184-1

10.1016/j.phytochem.2006.10.026

10.1021/acs.jnatprod.5b00179

Symonowicz M., 2012, Flavonoids and their properties to form chelate complexes, Biotechnology Food Science, 76, 35

10.1080/13880209.2016.1260597

10.1111/jfpe.12656

10.1271/bbb.60.909

10.1016/j.ifset.2008.11.006

10.1016/j.ifset.2007.04.014

10.3390/molecules23030579

10.1016/j.tifs.2005.12.004

10.1016/S1875-5364(17)30103-6

10.1021/jf062517l

10.3109/13880209.2010.523429

10.1016/j.seppur.2011.05.010

10.1007/s11947-018-2217-z