Improved elongation factor-1 alpha-based vectors for stable high-level expression of heterologous proteins in Chinese hamster ovary cells

Assaraf YG, Molina A, Schimke RT: Sequential amplification of dihydrofolate reductase and multidrug resistance genes in Chinese hamster ovary cells selected for stepwise resistance to the lipid-soluble antifolate trimetrexate. J Biol Chem. 1989, 264 (31): 18326-18334.

Running Deer J, Allison DS: High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1alpha gene. Biotechnol Prog. 2004, 20 (3): 880-889. 10.1021/bp034383r.

Zimmermann J, Hammerschmidt W: Structure and role of the terminal repeats of Epstein-Barr virus in processing and packaging of virion DNA. J Virol. 1995, 69 (5): 3147-3155.

Cho MS, Tran VM: A concatenated form of Epstein-Barr viral DNA in lymphoblastoid cell lines induced by transfection with BZLF1. Virology. 1993, 194 (2): 838-842. 10.1006/viro.1993.1327.

Cho MS, Chan SY: Vectors having terminal repeat sequence of Epstein-Barr virus. US Patent 6180108. 2001, Washington, DC: U.S. Patent and Trademark Office

Sun R, Spain TA, Lin SF, Miller G: Autoantigenic proteins that bind recombinogenic sequences in Epstein-Barr virus and cellular DNA. Proc Natl Acad Sci U S A. 1994, 91 (18): 8646-8650. 10.1073/pnas.91.18.8646.

Matsuo T, Heller M, Petti L, O′Shiro E, Kieff E: Persistence of the entire Epstein-Barr virus genome integrated into human lymphocyte DNA. Science. 1984, 226 (4680): 1322-1325. 10.1126/science.6095452.

Leenman EE, Panzer-Grumayer RE, Fischer S, Leitch HA, Horsman DE, Lion T, Gadner H, Ambros PF, Lestou VS: Rapid determination of Epstein-Barr virus latent or lytic infection in single human cells using in situ hybridization. Mod Pathol. 2004, 17 (12): 1564-1572. 10.1038/modpathol.3800228.

Hung SC, Kang MS, Kieff E: Maintenance of Epstein-Barr virus (EBV) oriP-based episomes requires EBV-encoded nuclear antigen-1 chromosome-binding domains, which can be replaced by high-mobility group-I or histone H1. Proc Natl Acad Sci U S A. 2001, 98 (4): 1865-1870. 10.1073/pnas.98.4.1865.

Urlaub G, Chasin LA: Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci U S A. 1980, 77 (7): 4216-4220. 10.1073/pnas.77.7.4216.

Derouazi M, Martinet D, Besuchet Schmutz N, Flaction R, Wicht M, Bertschinger M, Hacker DL, Beckmann JS, Wurm FM: Genetic characterization of CHO production host DG44 and derivative recombinant cell lines. Biochem Biophys Res Commun. 2006, 340 (4): 1069-1077. 10.1016/j.bbrc.2005.12.111.

Weiner M, Gackstetter T, Costa G, Bauer C, Kretz K: Site-directed Mutagenesis using PCR. Molecular Biology: Current Innovations and Future Trends. Edited by: Griffin A, Griffin H. 1995, Wymondham, Norfolk, U.K: Horizon Scientific Press

Orlova NA, Orlov AV, Vorobiev II: A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments. BMC Res Notes. 2012, 5: 303-10.1186/1756-0500-5-303.

Kozak M: An analysis of 5′-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987, 15 (20): 8125-8148. 10.1093/nar/15.20.8125.

Patterson GH, Knobel SM, Sharif WD, Kain SR, Piston DW: Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Biophys J. 1997, 73 (5): 2782-2790. 10.1016/S0006-3495(97)78307-3.

Ribeiro S, Mairhofer J, Madeira C, Diogo MM, Lobato da Silva C, Monteiro G, Grabherr R, Cabral JM: Plasmid DNA size does affect nonviral gene delivery efficiency in stem cells. Cell Reprogram. 2012, 14 (2): 130-137.

Wasley LC, Rehemtulla A, Bristol JA, Kaufman RJ: PACE/furin can process the vitamin K-dependent pro-factor IX precursor within the secretory pathway. J Biol Chem. 1993, 268 (12): 8458-8465.

Wajih N, Hutson SM, Owen J, Wallin R: Increased production of functional recombinant human clotting factor IX by baby hamster kidney cells engineered to overexpress VKORC1, the vitamin K 2,3-epoxide-reducing enzyme of the vitamin K cycle. J Biol Chem. 2005, 280 (36): 31603-31607. 10.1074/jbc.M505373200.

Bebbington CR, Hentschel CC: The use of vectors based on gene amplification for the expression of cloned genes in mammalian cells. DNA Cloning. Edited by: Glover D. 1987, San Diego: Academic, 163-188. Volume III