Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC)

Journal of Separation Science - Tập 31 Số 9 - Trang 1449-1464 - 2008
Zhigang Hao1, Baiming Xiao1, Naidong Weng2
1Research and Development, Bristol-Myers Squibb Company, NJ, USA.
2Global Preclinical Development, Johnson & Johnson Pharmaceutical Research and Development, NJ, USA

Tóm tắt

Abstract

The retention mechanism and chromatographic behavior for different polar analytes under hydrophilic interaction chromatography (HILIC) conditions have been studied by application of different mobile phases and stationary phases to various analytes at different temperatures. In addition to the commonly accepted mechanism of analyte liquid‐liquid partitioning between mobile phase and water‐enriched solvent layer which is partially immobilized onto the surface of the stationary phase, hydrogen‐bonding, hydrophobic interaction, and ion‐exchange interactions may also be involved. The predominant retention mechanism in HILIC separation is not always easily predictable. It can depend not only on the characteristics of the analytes but also on the selection of mobile and stationary phase compositions. The objective of this review is to evaluate the potential application of column temperature and mobile phase composition toward improving HILIC selectivity. The functional groups from analyte structures, stationary phase materials and organic mobile phase solvents will be highlighted.

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Thông tin xuất bản

Journal of Separation Science

Tập 31 Số 9

1449-1464

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