Impact of Potential Probiotic Lactobacillus Strains on Host Growth and Development in a Drosophila melanogaster Model
Tóm tắt
Lactobacillus paracasei SD1, Lactobacillus rhamnosus SD11, and Lactobacillus gasseri SD12 were proposed as potential probiotics for oral health. However, the effects of them on host physiology are still unknown. This study aimed to select strains that can promote host growth and development in monocolonized Drosophila model compared with axenic and the commercial Lactobacillus rhamnosus GG–treated flies. The morphogenesis and growth of axenic Drosophila melanogaster were assessed from embryo to adult stage when provided with each probiotic strain–supplemented food. The colonization and persistence of probiotic in fly gut were also evaluated. The results indicated that axenic condition caused the lowest adult weight and emergence rate. All probiotic groups had higher weight than axenic group. Lact. rhamnosus SD11 group presented high adult emergence rate equivalent to Lact. rhamnosus GG group, which is significantly higher than the others. However, Lact. gasseri SD12 group had significantly lower adult weight and emergence rate when compared with Lact. rhamnosus GG group. The gut probiotics levels were rapidly increased within the first day after receiving probiotics. After probiotic cessation, their number in gut decreased and was maintained at low level, except for Lact. gasseri SD12, which completely vanished since day one. In conclusion, Lact. paracasei SD1, Lact. rhamnosus SD11, and Lact. rhamnosus GG can affect morphogenesis and weight of flies when fed since immature stage and have short period gut colonization. The findings of this study could possibly imply comparable health-promoting effects between Lact. paracasei SD1 and Lact. rhamnosus SD11 to commercial strain in Drosophila model.
Tài liệu tham khảo
Food and Agriculture Organization of the United Nations (2006) Report of joint FAO/WHO export consultation on evaluation of health and nutrition properties of probiotics in food including powder milk with live lactic acid bacteria. In: Probiotics in food: health and nutritional properties and guidelines for evaluation. Food and Agriculture Organization of the United Nations: World Health Organization, Rome, pp. 1–33
Szajewska H, Kolodziej M, Gieruszczak-Bialek D, Skorka A, Ruszczynski M, Shamir R (2019) Systematic review with meta-analysis: Lactobacillus rhamnosus GG for treating acute gastroenteritis in children – a 2019 update. Alimentary Pharm Ther 49(11):1376–1384. https://doi.org/10.1111/apt.15267
Vanderhoof JA, Whitney DB, Antonson DL, Hanner TL, Lupo JV, Young RJ (1999) Lactobacillus GG in the prevention of antibiotic-associated diarrhea in children. J Pediatr 135(5):564–568. https://doi.org/10.1016/s0022-3476(99)70053-3
Onubi OJ, Poobalan AS, Dineen B, Marais D, McNeill G (2015) Effects of probiotics on child growth: a systematic review. J Health Popul Nutr 34:8. https://doi.org/10.1186/s41043-015-0010-4
Vendt N, Grunberg H, Tuure T, Malminiemi O, Wuolijoki E, Tillmann V, Sepp E, Korpela R (2006) Growth during the first 6 months of life in infants using formula enriched with Lactobacillus rhamnosus GG: double-blind, randomized trial. J Hum Nutr Diet 19(1):51–58. https://doi.org/10.1111/j.1365-277X.2006.00660.x
Teanpaisan R, Piwat S, Dahlen G (2011) Inhibitory effect of oral Lactobacillus against oral pathogens. Lett Appl Microbiol 53(4):452–459. https://doi.org/10.1111/j.1472-765X.2011.03132.x
Wannun P, Piwat S, Teanpaisan R (2014) Purification and characterization of bacteriocin produced by oral Lactobacillus paracasei SD1. Anaerobe 27:17–21. https://doi.org/10.1016/j.anaerobe.2014.03.001
Wannun P, Piwat S, Teanpaisan R (2016) Purification, characterization, and optimum conditions of Fermencin SD11, a bacteriocin produced by human orally Lactobacillus fermentum SD11. Appl Biochem Biotechnol 179(4):572–582. https://doi.org/10.1007/s12010-016-2014-y
Teanpaisan R, Piwat S (2014) Lactobacillus paracasei SD1, a novel probiotic, reduces mutans streptococci in human volunteers: a randomized placebo-controlled trial. Clin Oral Invest 18:857–862
Rungsri P, Akkarachaneeyakorn N, Wongsuwanlert M, Piwat S, Nantarakchaikul P, Teanpaisan R (2017) Effect of fermented milk containing Lactobacillus rhamnosus SD11 on oral microbiota of healthy volunteers: a randomized clinical trial. J Dairy Sci 100(10):7780–7787. https://doi.org/10.3168/jds.2017-12961
Teanpaisan R, Piwat S, Tianviwat S, Sophatha B, Kampoo T (2015) Effect of long-term consumption of Lactobacillus paracasei SD1 on reducing mutans streptococci and caries risk: a randomized placebo-controlled trial. Dent J 3(2):43–54. https://doi.org/10.3390/dj3020043
Piwat S, Sophatha B, Teanpaisan R (2015) An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity. Lett Appl Microbiol 61(1):98–105. https://doi.org/10.1111/lam.12434
Matos RC, Leulier F (2014) Lactobacilli-host mutualism: “learning on the fly”. Microb Cell Factories 13(Suppl 1):S6. https://doi.org/10.1186/1475-2859-13-s1-s6
Bai SP, Wu AM, Ding XM, Lei Y, Bai J, Zhang KY, Chio JS (2013) Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poult Sci 92(3):663–670. https://doi.org/10.3382/ps.2012-02813
Schwarzer M, Makki K, Storelli G, Machuca-Gayet I, Srutkova D, Hermanova P, Martino ME, Balmand S, Hudcovic T, Heddi A, Rieusset J, Kozakova H, Vidal H, Leulier F (2016) Lactobacillus plantarum strain maintains growth of infant mice during chronic undernutrition. Microbiome 351(6275):854–856
Kararli TT (1995) Comparison of the gastrointestinal anatomy, physiology, and biochemistry of humans and commonly used laboratory animals. Biopharm Drug Dispos 16(5):351–380. https://doi.org/10.1002/bdd.2510160502
Gomez E, Martin F, Nogacka AM, Salazar N, Alaez L, Alcorta E, Gueimonde M, De Los Reyes-Gavilan CG (2019) Impact of probiotics on development and behaviour in Drosophila melanogaster - a potential in vivo model to assess probiotics. Benef Microbes 10(2):179–188. https://doi.org/10.3920/bm2018.0012
Sabat D, Johnson EM, Abhinay A, Jayabalan R, Mishra M (2015) A protocol to generate germ free Drosophila for microbial interaction studies. Adv Tech Biol Med S1 001:1–6. https://doi.org/10.4172/2379-1764.S1-001
Piwat S, Teanpaisan R, Thitasomakul S, Thearmontree A, Dahlen G (2010) Lactobacillus species and genotypes associated with dental caries in Thai preschool children. Mol Oral Microbiol 25(2):157–164. https://doi.org/10.1111/j.2041-1014.2009.00556.x
Newell PD, Douglas AE (2014) Interspecies interactions determine the impact of the gut microbiota on nutrient allocation in Drosophila melanogaster. Appl Environ Microbiol 80(2):788–796
Storelli G, Defaye A, Erkosar B, Hols P, Royet J, Leulier F (2011) Lactobacillus plantarum promotes Drosophila systemic growth by modulating hormonal signals through TOR-dependent nutrient sensing. Cell Metab 14:403–414
Kasozi KI, Bukenya A, Eze ED, Kasolo J, Tayebwa DS, Ssempijja F, Suubo J, Tamale A, Echoru I, Ntulume I, Onkoba SK, Micheni LN, Ayikobua ET, Asiimwe OH, Kalange M (2019) Low concentrations of Lactobacillus rhamnosus GG (Yoba®) are safe in male Drosophila melanogaster. BMC Res Notes 12(1):269. https://doi.org/10.1186/s13104-019-4297-x
Shin SC, Kim SH, You H, Kim B, Kim AC, Lee KA, Yoon JH, Ryu JH, Lee WJ (2011) Drosophila microbiome modulates host developmental and metabolic homeostasis via insulin signaling. Science 334:670–674. https://doi.org/10.1126/science.1212782
McFarland LV, Evans CT, Goldstein EJC (2018) Strain-specificity and disease-specificity of probiotic efficacy: a systematic review and meta-analysis. Front Med 5:124. https://doi.org/10.3389/fmed.2018.00124
Baldwin S, Hughes RJ, Hao Van TT, Moore RJ, Stanley D (2018) At-hatch administration of probiotic to chickens can introduce beneficial changes in gut microbiota. PLoS One 13(3):e0194825. https://doi.org/10.1371/journal.pone.0194825
Tuohy KM, Pinart-Gilberga M, Jones M, Hoyles L, McCartney AL, Gibson GR (2007) Survivability of a probiotic Lactobacillus casei in the gastrointestinal tract of healthy human volunteers and its impact on the faecal microflora. J Appl Microbiol 102(4):1026–1032. https://doi.org/10.1111/j.1365-2672.2006.03154.x
Fetissov SO (2017) Role of the gut microbiota in host appetite control: bacterial growth to animal feeding behaviour. Nat Rev Endocrinol 13(1):11–25
Grewal SS (2012) Controlling animal growth and body size - does fruit fly physiology point the way? F1000 Biol Rep 4:12. doi:https://doi.org/10.3410/b4-12
Brummel T, Ching A, Seroude L, Simon AF, Benzer S (2004) Drosophila lifespan enhancement by exogenous bacteria. Proc Natl Acad Sci U S A 101(35):12974–12979. https://doi.org/10.1073/pnas.0405207101
Aureli P, Capurso L, Castellazzi AM, Clerici M, Giovannini M, Morelli L, Poli A, Pregliasco F, Salvini F, Zuccotti GV (2011) Probiotics and health: an evidence-based review. Pharmacol Res 63(5):366–376. https://doi.org/10.1016/j.phrs.2011.02.006