Immunostimulating Polysaccharides from Panax notoginseng

Pharmaceutical Research - Tập 13 Số 8 - Trang 1196-1200 - 1996
Gao, Hua1, Wang, Fengzhen1, Lien, Eric J.1, Trousdale, Melvin D.2
1Department of Pharmaceutical Sciences, School of Pharmacy, University of Southern California, LA
2Doheny Eye Institute, School of Medicine, University of Southern California, LA

Tóm tắt

Purpose. The main purpose of this study is to prepare and characterize polysaccharides from Panax notoginseng, investigate their effects on immune system in vitro in order to find new immunostimulants for the prevention and supporting treatment of infection and immunodeficiency related diseases. Methods. Polysaccharides were extracted with aqueous solution, separated with column chromatography. Their anticomplementary activities were investigated by using human serum and antibody-sensitized sheep red blood cells. Interferon-γ and tumor necrosis factor inductive activities were studied by using isolated mouse spleen lymphocytes and peritoneal macrophages, respectively. Results. Four polysaccharides, homogeneous in gel-filtration chromatography, were prepared and designated PF3111, PF3112, PBGA11, and PBGA12. Component sugar analysis revealed that they are heteroglycans with MWs ranging from 37 kD to 760 kD, composed of glucose, galactose, arabinose, mannose, and xylose in different molar ratios. Fraction PBGA12 has the most anticomplementary activity which is mediated through both alternative and classical pathways. All the polysaccharides except PBGA11 induced the production of interferon-γ in the presence of concanavalin A. They induced the production of significant amount of TNF-α in cell cultures. Conclusions. The polysaccharides from P. notoginseng have immunostimulating activities in vitro.

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Tài liệu tham khảo

citation_journal_title=Int. J. Oriental Med.; citation_title=Higher plant polysaccharides and their pharmacological activities; citation_author=E. J. Lien, H. Gao.; citation_volume=15; citation_publication_date=1990; citation_pages=123-140; citation_id=CR1

citation_journal_title=Prog. Drug Res.; citation_title=Fungal metabolites and Chinese herbal medicine as immunostimulants; citation_author=E. J. Lien.; citation_volume=34; citation_publication_date=1990; citation_pages=395-420; citation_id=CR2

citation_title=Oriental material Medica. A concise guide; citation_publication_date=1986; citation_id=CR3; citation_author=H.-Y. Hsu.; citation_publisher=Oriental Healing Art Institute.

citation_journal_title=Anal. Chem.; citation_title=Colorimetric method for determination of sugars and related substances; citation_author=M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, F. Smith. ; citation_volume=28; citation_publication_date=1956; citation_pages=350; citation_id=CR4

citation_journal_title=Anal. Biochem.; citation_title=Analysis of reducing sugars as their chromophoric hydrazones by high performance liquid chromatography; citation_author=K. Muramoto, R. Goto, H. Kamiya.; citation_volume=162; citation_publication_date=1987; citation_pages=435-442; citation_id=CR5

citation_journal_title=Anal. Biochem.; citation_title=Fluorescent hydrazides for the high performance liquid chromatographic determination of biological carbonyls; citation_author=J. M. Anderson.; citation_volume=152; citation_publication_date=1986; citation_pages=146-153; citation_id=CR6

citation_journal_title=Biol. Pharm. Bull.; citation_title=Arabinogalactan core structure and immunological activities of Ukonan C, an acidic polysaccharide from the Rhizome of Curcuma longa; citation_author=R. Gonda, M. Tomoda, N. Ohara, K. Takada.; citation_volume=16; citation_publication_date=1993; citation_pages=235-238; citation_id=CR7

citation_title=The interferon system; citation_publication_date=1979; citation_id=CR8; citation_author=W. E. Stewart.; citation_publisher=Springer-Verlag New York-Wien

citation_journal_title=Int. J. Oriental Med.; citation_title=Immunostimulating polysaccharides from Viola yedoensis ; citation_author=H. Gao, F. Z. Wang, C. S. Hsu, E. J. Lien, M. D. Trousdale. ; citation_volume=21; citation_publication_date=1996; citation_pages=1-9; citation_id=CR9

citation_journal_title=Planta. Med.; citation_title=Sanchinan-A, a reticuloendothelial system activating arabinogalactan from Sanchiginseng (root of Panax notoginseng); citation_author=K. Ohtani, K. Mizutani, S. Hatono, R. Kasai, R. Sumino, T. Shioto, M. Ushijima, J. Zhou, T. Fuwa.; citation_volume=53; citation_publication_date=1987; citation_pages=166-169; citation_id=CR10

citation_journal_title=Microbiol. Immunol.; citation_title=The mode of production of endotoxin-induced interferon in rabbit tissue cells. I. Development of priming by pretreatment with interferon; citation_author=H. Hashimoto, N. Shibukawa, Y. Kojima.; citation_volume=22; citation_publication_date=1978; citation_pages=673-681; citation_id=CR11

citation_journal_title=J. Immunol.; citation_title= Salmonella typhimurium induces IFN-γ production in murine splenocytes. role of natural killer cells and macrophages; citation_author=L. Ramarathinam, D. W. Niesel, G. R. Klimpel.; citation_volume=150; citation_publication_date=1993; citation_pages=3973-3981; citation_id=CR12

E. J. Lien, L. L. Lien, and H. Gao. Structure-system-activity relationship analysis of drug disposition. 10th European Symposium on Structure-Activity Relationships: QSAR and Molecular Modelling. September 4–9, Barcelona, Spain, 1994.

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Pharmaceutical Research

Tập 13 Số 8

1196-1200

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