Immunomodulatory effect of human adipose tissue‐derived adult stem cells: comparison with bone marrow mesenchymal stem cells

British Journal of Haematology - Tập 129 Số 1 - Trang 118-129 - 2005
Bénédicte Puissant-Lubrano1,2, Corinne Barreau3,4, Philippe Bourin5,3, C Clavel2,3, Jill Corre4, Christine Bousquet2, Christine Taureau2, Béatrice Cousin4, M. Abbal1,2, Patrick Laharrague6,4, Luc Pénicaud4, Louis Casteilla4, Antoine Blancher1,2
1Laboratoire d'Immunogénétique Moléculaire, Faculté de médecine Toulouse-Rangueil, Bâtiment A2, 133 Route de Narbonne, Toulouse Cedex 04
2Laboratoire d'Immunologie, Hôpital Rangueil, 1 avenue Jean Poulhès, TSA 50032, Toulouse cedex 9
3These authors contributed equally to this study
4UMR-CNRS 5018, IFR31, Hôpital Rangueil, 1 avenue Jean Poulhès, TSA 50032, Toulouse cedex 9
5Service de thérapie cellulaire Etablissement Français du Sang, Pyrénées Méditerranée, Site de Toulouse
6Laboratoire d'Hématologie, Hôpital Rangueil, 1 avenue Jean Poulhès, TSA 50032, Toulouse cedex 9, France

Tóm tắt

SummaryLike mesenchymal stem cells from bone marrow (BM‐MSCs), adipose tissue‐derived adult stem cells (ADAS cells) can differentiate into several lineages and present therapeutical potential for repairing damaged tissues. The use of allogenic stem cells can enlarge their therapeutical interest, provided that the grafted cells could be tolerated. We investigate here, for the first time, the immunosuppressive properties of ADAS cells compared with the well‐characterized immunosuppressive properties of BM‐MSCs. ADAS cells did not provoke in vitro alloreactivity of incompatible lymphocytes and, moreover, suppressed mixed lymphocyte reaction (MLR) and lymphocyte proliferative response to mitogens. The impairment of inhibition when ADAS cells and BM‐MSCs were separated from lymphocytes by a permeable membrane suggests that cell contact is required for a full inhibitory effect. Hepatocyte growth factor is secreted by both stem cells but, similar to interleukin‐10 and transforming growth factor‐β (TGF‐β), the levels of which were undetectable in supernatants of MLR inhibited by ADAS cells or BM‐MSCs, it did not seem implicated in the stem cell suppressive effect. These findings support that ADAS cells share immunosuppressive properties with BM‐MSCs. Therefore, ADAS cell‐based reconstructive therapy could employ allogenic cells and because of their immunosuppressive properties, ADAS cells could be an alternative source to BM‐MSCs to treat allogenic conflicts.

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British Journal of Haematology

Tập 129 Số 1

118-129

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