Immunolocalization of extracellular matrix proteins and collagen synthesis in first‐trimester human decidua
Tóm tắt
The extracellular matrix (ECM) of first‐trimester human decidua was examined with indirect immunofluorescence using affinity‐purified antibodies to human collagen types I, III, IV, V, laminin, and fibronectin. In addition, the validity of the classification “mesenchymal‐epithelioid” for differentiated decidual cells was addressed using antibodies to the intermediate filament proteins, vimentin, a mesenchymal marker, and keratin, an epithelial marker. Biosynthesis of extracellular matrix components was examined by radiolabeling of decidual explants in culture with 3H‐proline, followed by immunoprecipitations of synthesized proteins with collagen type‐specific antibodies. Immunofluorescence showed decidual cells are embedded in an extensive network of collagen types I and III, and intracytoplasmic staining suggested synthesis of these collagens by the decidual cells. Collagen type IV and laminin localized in the external lamina which surrounds the differentiated decidual cell, and some fluorescence was evident in the peripheral cytoplasm. Immunoreactive collagen type V was observed in close association with the external lamina and in the peridecidual matrix. Fibronectin localized throughout the decidual ECM and in fibrillar and punctuate patterns in the decidual cell cytoplasm. Differentiated decidual cells retained a “mesenchymal” intermediate filament cytoskeleton containing an abundance of vimentin filaments, but very few, if any, keratin filaments. Collagen types I, III, V, and to a lesser extent, IV, were immunoprecipitated from the medium of decidual explants after 24 hours of culture, demonstrating in vitro synthesis and secretion of these collagens by first trimester human decidua.
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Tài liệu tham khảo
Azab I., 1972, Decidual cell production of human placental fibrinoid, Obstet. Gynecol., 40, 186
Furuto D. K., 1981, Characterization of a novel collagen chain in human placenta and its relation to AB collagen, Biochemistry, 18, 3815
Little C. D., 1982, Masking of extracellular collagen and the codistribution of collagen and fibronectin during matrix formation by cultured embryonic fibroblasts, J. Cell Sci., 55, 35, 10.1242/jcs.55.1.35
Miller E. J., 1982, Methods in Enzymology, 33
Moe N., 1969, The deposits of fibrin and fibrin‐like materials in the basal plate of the normal human placenta, Acta Pathol. Microbiol. Scand., 75, 1
Montfort I., 1961, Studies on uterine collagen during pregnancy and puerperium, Lab. Invest., 10, 1240
Novak E. R., 1970, Textbook of Gynecology, 53
Timpl R., 1979, Laminin‐A glycoprotein from basement membranes, J. Biol. Chem., 254, 9993, 10.1016/S0021-9258(19)83607-4
Wallart J., 1936, Les vegetations deciduales ectopiques pendant et en dehors de la grossesse, en particulier celles de l'ovaire, Gynecol. Obstet., 33, 134
Wewer U. M., 1985, Immunochemical and ultrastructural assessment of the nature of the pericellular basement membrane of human decidual cells, Lab. Invest., 53, 624
Wolfe J. M., 1942, The fibrous connective tissue of the artificially induced maternal placenta in the rat with particular reference to the relationship between reticulum and collagen, Am. J. Pathol., 18, 431