Immunolocalization of extracellular matrix proteins and collagen synthesis in first‐trimester human decidua

Wiley - Tập 218 Số 4 - Trang 402-415 - 1987
Lydia L. Kisalus1, John C. Herr1, Charles D. Little1
1Department of Anatomy and Cell Biology, Medical Center, University of Virginia, Charlottesville, VA 22908

Tóm tắt

AbstractThe extracellular matrix (ECM) of first‐trimester human decidua was examined with indirect immunofluorescence using affinity‐purified antibodies to human collagen types I, III, IV, V, laminin, and fibronectin. In addition, the validity of the classification “mesenchymal‐epithelioid” for differentiated decidual cells was addressed using antibodies to the intermediate filament proteins, vimentin, a mesenchymal marker, and keratin, an epithelial marker. Biosynthesis of extracellular matrix components was examined by radiolabeling of decidual explants in culture with 3H‐proline, followed by immunoprecipitations of synthesized proteins with collagen type‐specific antibodies. Immunofluorescence showed decidual cells are embedded in an extensive network of collagen types I and III, and intracytoplasmic staining suggested synthesis of these collagens by the decidual cells. Collagen type IV and laminin localized in the external lamina which surrounds the differentiated decidual cell, and some fluorescence was evident in the peripheral cytoplasm. Immunoreactive collagen type V was observed in close association with the external lamina and in the peridecidual matrix. Fibronectin localized throughout the decidual ECM and in fibrillar and punctuate patterns in the decidual cell cytoplasm. Differentiated decidual cells retained a “mesenchymal” intermediate filament cytoskeleton containing an abundance of vimentin filaments, but very few, if any, keratin filaments. Collagen types I, III, V, and to a lesser extent, IV, were immunoprecipitated from the medium of decidual explants after 24 hours of culture, demonstrating in vitro synthesis and secretion of these collagens by first trimester human decidua.

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Thông tin xuất bản

Wiley

Tập 218 Số 4

402-415

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