Immunohistochemical demonstration of parietal epithelial cells and macrophages in human proliferative extra-capillary lesions

C. Guettier1, D. Nochy1, C. Jacquot1, C. Mandet1, J. P. Camilleri1, J. Bariety1
1Inserm U 28, Hôpital Broussais, Paris Cedex, France

Tóm tắt

The cellular composition of crescents in diffuse crescentic glomerulonephritis is still controversial. Ten renal biopsies were studied on serial sections by using antikeratin antibodies as specific markers of epithelial cells of Bowman's capsule and both anti-macrophage and anti-lymphocyte antibodies. Semiautomatic morphometry showed that cellular crescents consisted of epithelial ceils of Bowman's capsule (24–61%), of macrophages (19–34%) and of unlabelled cells (12–53%). In each biopsy, parietal epithelial cells outnumbered macrophages within crescents.

Tài liệu tham khảo

Atkins RC, Glasgow EF, Holdsworth SR, Matthews FE (1976) The macrophage in human rapidly progressive glomerulonephritis. Lancet i.:830–832

Atkins RC, Hancock WW, Stwo J, Becker GJ, Thomson NM, Glasgow EF (1981) Macrophages identification in human and experimental glomerulonephritis. In Proceedings of the 8th International Congress of Nephrology. Advances in Basic and Clinical Nephrology, Zurukzoglu W, Papadimitriou M, Pyrpasopoulos M, Sion M, Zamboulis T (eds), p 865–871

Bacani RA, Velasquez F, Kanter A, Pirani CL, Pollack VE (1968) Rapidly progressive (non-streptococcal) glomerulonephritis. Ann Intern Med 69:463–485

Charron DJ (1981) Ia antigens on human lymphocytes: cellular expression and molecular structure: an overview: Immunol Commun 10:293–311

Heptinstall RH (1966) Pathology of the Kidney. Ed 1rst, Churchill, London, p 275–286

Winchester RJ, Kunkel HG (1979) The human Ia system. Adv Immunol 28:222–292