Immunohistochemical characterization of inflammatory cells associated with advanced atherosclerosis
Tóm tắt
During repair of 12 atherosclerotic abdominal aortic aneurysms, fresh samples of aneurysm wall were obtained. Histology confirmed the presence of advanced atherosclerosis associated with medial thinning and a variable aortic adventitial chronic inflammatory cell infiltrate. Monoclonal antibodies were used to identify the inflammatory cells throughout the aortic wall. The majority of lymphocytes in the aortic adventitia were B‐cells. B‐cells were not present in atheromatous plaques. T‐cells, predominantly T‐helper cells, were found in atheromatous plaques and in aortic adventitia. The majority of lymphocytes and macrophages in aortic adventitia and most vascular endothelial cells were HLA‐DR positive. Ki‐67 staining was found in B‐cells and T‐helper cells, indicating that these cells were proliferating. Occasional lymphocytes were BerH2 positive, indicating that some lymphocytes were activated. These findings suggest that chronic periaortitis is an active, immunoiogically mediated, local complication of advanced human atherosclerosis.
Từ khóa
Tài liệu tham khảo
Mitchinson MJ., 1984, The vasculitis syndromes., Rec. Adv. Histopathol., 12, 223
Bloor K, 1979, Aneurysms of the abdominal aorta., Br. J. Hosp. Med., 21, 568
Rose AG, 1981, Inflammatory variant of abdominal atherosclerotic aneurysm., Arch. Pathol., 105, 409
Ball RV, 1985, The production of insoluble lipid by mouse peritoneal macrophages in vitro., Br. J. Exp. Pathol., 65, 710
Parums DV, 1985, Autoallergy in atherosclerosis., J. Pathol., 146, 245A
Parums DV, 1990, Serum antibodies to oxidized LDL and ceroid in chronic periaortitis., Arch. Pathol. Lab. Med., 114, 383
Parums DV, 1990, Characterization of inflammatory cells in a patient with chronic periaortitis., Am. J. Cardiovasc. Pathol.
Pulford KAF, 1987, Leucocyte Typing III, 828
Smith SH, 1986, Functional subsets of human helper‐inducer cells defined by a new monoclonal antibody, UCHL, I., Immunology, 58, 63
Stein H, 1982, The normal malignant germinal center., Clin. Haematol., 11, 531, 10.1016/S0308-2261(82)80005-2
Ghosh AK, 1984, Immunocytochemical characterization of monoclonal anti‐HLA‐DR antibodies., Disease Markers, 2, 223
Gatter KC, 1984, The use of monoclonal antibodies in histopathological diagnosis., Rec. Adv. Histopathol., 12, 35
Bach MA, 1981, The use of monoclonal anti‐T cell antibodies to study T cell imbalances in human disease., Clin. Exp. Immunol., 45, 449
Blue ML, 1985, Co‐expression of T4 and T8 on peripheral blood T cells demonstrated by two colour fluorescence flow cytometry., J. Immunol., 134, 2281, 10.4049/jimmunol.134.4.2281
Jonasson L, 1985, Expression of class II transplantation antigen on vascular smooth muscle cells in human atherosclerosis, J. Clin. Pathol., 76, 125
Hansson GK, 1989, Detection of activated T lymphocytes in the human atherosclerotic plaque., Am. J. Pathol., 135, 169
Wall AC, 1989, Atherosclerotic lesions in humans. In‐situ immunophenotypic analysis suggesting an immune mediated response, Lab. Invest., 61, 166
Leibovich SJ, 1976, A macrophage‐dependent factor that stimulates the proliferation of fibroblasts in vitro., Am. J. Pathol., 84, 501