Immunogenicity of the Shigella flexneri Serotype Y (SFL124) Vaccine Strain Expressing Cloned Glucosyl Transferase Gene of Converting Bacteriophage SfX

Microbiology and Immunology - Tập 39 Số 7 - Trang 467-472 - 1995
Pham thi Huan1,2, Rona Taylor2, Alf A. Lindberg1, Naresh K. Verma2
1Department of Clinical Bacteriology Karolinska Institute, Huddinge Hospital S‐141 86 Huddinge Sweden
2Division of Biochemistry and Molecular Biology, Faculty of Science, School of Life Sciences, The Australian National University Canberra, ACT 0200 Australia

Tóm tắt

AbstractThe glucosyl transferase gene (gtr) from bacteriophage phage X (SfX) caused partial conversion of serotype Y (group antigen 3, 4) to X (group antigen 7, 8) when introduced into a candidate vaccine strain of Shigella flexneri serotype Y (SFL124). The gtr gene caused conversion of O‐antigens but did not eliminate the adsorption of the corresponding phage SfX. The hybrid strain expressing both group antigens 7, 8 and 3, 4 showed 75% protection when immunized guinea pigs were challenged with a wild‐type S. flexneri serotype X strain. No protection was observed against serotype Y challenge, although group antigen 3, 4 was detected in the LPS of the hybrid strain. This suggests the importance of O‐antigen immunity in the host defense against shigellosis.

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Tài liệu tham khảo

Bennish M.L. andWojtyniak B.J.1991.Mar‐Apr 13 Suppl 4. Mortality due to shigellosis: community and hospital data.Rev. Infect. Dis.245–251.

Carlin N.I.A., 1983, Monoclonal antibodies specific for O‐antigenic polysaccharides of Shigella flexneri: clones binding to II, II: 3,4 and 7,8 epitopes, J. Clin. Microbiol., 18, 1183, 10.1128/jcm.18.5.1183-1189.1983

Carlin N.I.A., 1987, Monoclonal antibodies specific for Shigella flexneri lipopolysaccharides: clones binding to type IV, V and type VI antigens, group antigen 3, 4 antigen and an epitope common to all S. flexneri and S. dysenteriae type 1 strains, Infect. Immun., 55, 1412, 10.1128/iai.55.6.1412-1420.1987

10.1016/0378-1119(91)90295-M

10.1093/oxfordjournals.aje.a116134

Giammanco G., 1968, Conversion lysogenique de caracteres antigeniques de Shigella flexneri (antigene II de type et complexe 7, 8 de group), Ann. Inst. Pasteur, 114, 63

Hartman A.B., 1991, Small animal model to measure efficacy and immunogenicity of Shigella vaccine strains, Infect. Immun., 59, 4075, 10.1128/iai.59.11.4075-4083.1991

10.1097/00006454-199203000-00009

10.1016/0264-410X(92)90007-7

10.1111/j.1432-1033.1978.tb20963.x

10.1111/j.1432-1033.1978.tb20963.x

Lindberg A.A., 1978, Interaction between bacteriophage Sf6 and Shigella flexneri, J. Virol., 27, 38, 10.1128/jvi.27.1.38-44.1978

10.1016/0882-4010(90)90030-T

10.1016/S0264-410X(88)80018-5

10.1016/0264-410X(93)90015-P

Mikhail I.A., 1990, Epidemiology of bacterial pathogens associated with infectious diarrhea in Djibouti, J. Clin. Microbiol., 28, 956, 10.1128/jcm.28.5.956-961.1990

Penny J.H., 1983, Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver‐stained polyacrylamide gels, J. Bacteriol., 154, 269, 10.1128/jb.154.1.269-277.1983

10.1007/978-1-4684-5490-1_11

Sereny B., 1957, Experimental keratoconjunctivitis shigellosa, Acta Microbiol. Acad. Sci. Hung., 4, 367

10.1099/00222615-23-4-289

10.1073/pnas.76.9.4350

10.1016/0378-1119(93)90702-5

10.1111/j.1365-2958.1991.tb01827.x

Zaman K., 1991, Surveillance of shigellosis in rural Bangladesh: a ten years review, J. Pak. Med. Assoc., 41, 57

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Microbiology and Immunology

Tập 39 Số 7

467-472

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