Immunoassay for Transthyretin Variants associated with Amyloid Neuropathy

Scandinavian Journal of Immunology - Tập 38 Số 2 - Trang 177-182 - 1993
Paulo Costa1, A. Teixeira1, Maria João Saraiva2
1Centro de Estudos de Paramiloidose do Instituto National de Saúde, Hospital de Sto, António, Porto, Portugal
2lnstituto de Ciências Biomédcas Abel Salazar, Porto, Portugal

Tóm tắt

An anti‐transthyretin (TTR) mouse monoclonal antibody (88.6.FD6)of IgG1 subclass, obtained using as immunogen TTR from the serum of a patient with familial amyloidotic polyneuropathy, was found to bind to sera from carriers of several amyloidogenic TTR variants associated with peripheral neuropathy, but not to normal sera or sera from carriers of non‐pathogenic or cardiomyopathic variants, in an ELISA performed under special conditions. Further characterization suggests that it recognizes an epitope near the N‐terminal side of the TTR monomer. We propose that this epitope is exposed in amyloid and could be implicated in fibril deposition in the peripheral nervous system.

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