Immunization of mice and baboons with the recombinant Sm28GST affects both worm viability and fecundity after experimental infection with Schistosoma mansoni

Parasite Immunology - Tập 13 Số 5 - Trang 473-490 - 1991
Denis Boulanger1, Gregory J. Reid2, R. F. Sturrock3, Isabelle Wolowczuk1, Jean-Marc Balloul1, Délphine Grezel1, Raymond J. Pierce1, Michael F. Otieno2, Sylviane Guérret4, A. GRIMAUD4, André M. Deelder5, A Capron1
1Centre d'Immunologie el de Biologic Parasitaire, Unité Mixte INSERM 167-CNRS 624, Institut Pasteur, 59019 Lille Cedex. France
2Institute of Primate Research, Nairobi, Kenya
3Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, London, UK
4Centre de Microscopic Electronique et de Pathologie Ultrastructurale, Institut Pasteur, Lyon Cedex, France
5Department of Pathology, Immunology Division, Cambridge University, UK

Tóm tắt

Summary A member of the glutathione S‐transferase family. Sm28GST has previous! demonstrated a good ability to protect rodents against experimental infection with Schistosoma mansoni. In order lo evaluate its efficacy in a model closer to man, two different protocols of immunization with recombinant Sm28GST were tested on baboons in a large‐scale trial. Three injections in the presence of aluminium hydroxide as adjuvant resulted in a significant 38% reduction in the adult worm burden together with a trend for a lower percentage of inflammatory tissue in the liver. Individual levels of protection, ranging from 0 to 80%, underlined the heterogeneity of the immune response to this purified molecule in outbred primates. On the other hand, two injections of Sm28GST in the presence of aluminium hydroxide and Bordetella pertussis reduced female schistosome fecundity by 33%, with a more pronounced effect (66%) on faecal egg output; there was also a trend, in this protocol, for decrease of the mean granuloma surface in the liver. Individual anti‐Sm28GST IgG antibodies were apparently unrelated to levels of immunity, but there was partial evidence that cytophilic IgE might play a role in the immune mechanisms affecting worm viability, but not fecundity. In the mouse model, Sm28GST vaccination resulted in a lower hatching ability of tissue eggs recovered from immunized mice whereas passive transfer of specific anti‐Sm28GST T‐lymphocytes, one day before infection, significantly reduced the number of eggs in the liver of mice. We propose that different protocols of immunization with a recombinant molecule can impede Schistosoma mansoni worm viability and fecundity, but can also affect miracidium

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Parasite Immunology

Tập 13 Số 5

473-490

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