Immunization against malaria with a recombinant protein

Parasite Immunology - Tập 16 Số 2 - Trang 63-67 - 1994
Irene T. Ling1, Solabomi A. Ogun2, Anthony A. Holder2
1Division of Parasitology, National Institute for Medical Research, Mill Hill, London, UK
2Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK

Tóm tắt

SUMMARYWe have expressed in bacteria the C‐terminal part of Plasmodium yoelii merozoite surface protein‐1 (MSP1) containing the two epidermal growth factor‐like domains. The protein, either alone or fused to glutathione Stransferase, was highly effective as a vaccine and protected mice against challenge infection. Reduction and alkylation abolished the protection obtained with the protein. This shows for the first time the absolute requirement of the disulphide‐bonded conformation for immunogenicity. In a short term experiment, mice were protected against a massive challenge. The immunity was effective at the time of merozoite release/reinvasion. Recombinant protein based on this part of MSP1 may be suitable as a vaccine against malaria.

Từ khóa


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Parasite Immunology

Tập 16 Số 2

63-67

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