Immune dependence of schistosomicidal chemotherapy: an ultrastructural study of Schistosoma mansoni adult worms exposed to praziquantel and immune serum in vivo

Parasite Immunology - Tập 12 Số 3 - Trang 321-334 - 1990
J Modha1,2, José Roberto Lambertucci2,3, M. J. Doenhoff2,4,5, Diane J. McLaren6,4,5
1*Department of Biochemistry, University of Leicester, University Road, Leicester LEI 7RH, UK
2Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Winches Farm Laboratories, 395 Hatfield Road, St Albans, Herts AL4 OXQ, UK
3†Faculty of Medicine, Federal University of Minas Gerais, 30000-Belo Horizonte (MG), Brazil.
4Dr D.J.McLaren
5School of Biological Sciences, University College of North Wales, Bangor, Gwynedd, UK LL57 2UW
6Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK

Tóm tắt

Summary This study examines the immune‐dependence of praziquantel (PZQ) for the treatment of Schistosomiasis mansoni in mice. We have shown elsewhere from worm recovery data that the efficacy of PZQ is significantly enhanced when mice are treated concomitantly with antisera raised against antigens released from adult schistosomes, even though such antisera show no intrinsic helminthotoxic activity (Doenhoff et al. 1987, DoenhofT, Modha & Lambertucci 1988). Moreover, indirect immunofluorescence assays have shown that male worms exposed to the dual treatment regime in vivo bind antiserum to their dorsal surfaces in a pattern that seems to follow the outline of the dorsal tubercles. Scanning and transmission electron microscopy have now been used to further define the features of damage inflicted upon worms through exposure to antiserum alone, drug alone, or the two treatments in combination. Such investigations revealed that the antiserum induces a classical membrane repair process in worms of both sexes, but little other damage. PZQ causes the formation of spherical protuberances on the dorsal tubercles of male worms, while the dual treatment regime induces both kinds of damage in male schistosomes, but with much enhanced severity. The protuberances show evidence of explosion and some regions of the tegument become completely destroyed.Regions other than the dorsal surfaces of the male worms do not exhibit comparable trauma, and neither do the females. These data are discussed in relation to the known schistosomicidal activity of PZQ, the notion that male and female worms exhibit regional and sexual differences in susceptibility, documerited evasive strategies of the parasite and the interdependence of immuno‐ and chemotherapy.

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Tài liệu tham khảo

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Parasite Immunology

Tập 12 Số 3

321-334

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