Imaging applications of synchrotron X‐ray phase‐contrast microtomography in biological morphology and biomaterials science. I. General aspects of the technique and its advantages in the analysis of millimetre‐sized arthropod structure

Journal of Microscopy - Tập 227 Số 1 - Trang 51-71 - 2007
Oliver Betz1, Ulrike G. K. Wegst2,3, Daniela Weide1, Michael Heethoff1, Lukas Helfen4,5, Wah-Keat Lee6, Peter Cloetens4
1Zoologisches Institut der Universität, Abteilung Evolutionsbiologie der Invertebraten, Auf der Morgenstelle 28E, D-72076 Tübingen, Germany
2Lawrence Berkeley National Laboratory, Materials Sciences Division, Berkeley, CA 94720, USA
3Max-Planck-Institut für Metallforschung, Heisenbergstr. 3, D-70569 Stuttgart, Germany
4European Synchrotron Radiation Facility, B.P. 220, RF-38043 Grenoble, France
5Forschungszentrum Karlsruhe, Institut für Synchrotronstrahlung (ISS/ANKA), D-76133 Karlsruhe, Germany
6Argonne National Laboratory, Advanced Photon Source, X-ray Science Division, Argonne, IL 60439, USA

Tóm tắt

SummarySynchrotron‐generated X‐rays provide scientists with a multitude of investigative techniques well suited for the analysis of the composition and structure of all types of materials and specimens. Here, we describe the properties of synchrotron‐generated X‐rays and the advantages that they provide for qualitative morphological research of millimetre‐sized biological organisms and biomaterials. Case studies of the anatomy of insect heads, of whole microarthropods and of the three‐dimensional reconstruction of the cuticular tendons of jumping beetles, all performed at the beamline ID19 of the European Synchrotron Radiation Facility (ESRF), are presented to illustrate the techniques of phase‐contrast tomography available for anatomical and structural investigations. Various sample preparation techniques are described and compared and experimental settings that we have found to be particularly successful are given. On comparing the strengths and weaknesses of the technique with traditional histological thin sectioning, we conclude that synchrotron radiation microtomography has a great potential in biological microanatomy.

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Journal of Microscopy

Tập 227 Số 1

51-71

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