Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Tóm tắt
We introduce a method for optically imaging intracellular proteins at nanometer spatial resolution. Numerous sparse subsets of photoactivatable fluorescent protein molecules were activated, localized (to ∼2 to 25 nanometers), and then bleached. The aggregate position information from all subsets was then assembled into a superresolution image. We used this method—termed photoactivated localization microscopy—to image specific target proteins in thin sections of lysosomes and mitochondria; in fixed whole cells, we imaged vinculin at focal adhesions, actin within a lamellipodium, and the distribution of the retroviral protein Gag at the plasma membrane.
Từ khóa
Tài liệu tham khảo
Materials and methods are available as supporting material on Science Online.
This project was supported in part by the Intramural Program of NICHD and the NIH Intramural AIDS Targeted Antiviral Program (IATAP). We thank J. Wiedenmann and U. Nienhaus of the University of Ulm for the gift of a cDNA encoding the EosFP.