Image restoration in sets of noisy electron micrographs
Proceedings IEEE International Symposium on Biomedical Imaging - Trang 745-748
Tóm tắt
The imaging process in a transmission electron microscope (TEM) produces a number of artifacts including the contrast transfer function (CTF) and envelope functions. In addition, when electron cryomicroscopy is performed for purposes of single particle reconstruction, signal to noise ratios are very low, generally peaking between 0.1 and 1.0, and averaging 0.01 to 0.1. We apply a semi-empirical model of the microscope artifacts to optimally correct data used for 3D single particle reconstructions.
Từ khóa
#Image restoration #Transmission electron microscopy #Signal to noise ratio #Fourier transforms #Image reconstruction #Energy resolution #Signal resolution #Coordinate measuring machines #Educational institutions #Biomedical imagingTài liệu tham khảo
10.1006/jsbi.2001.4330
10.1006/jmbi.2001.5133
10.1006/jsbi.1999.4168
10.1006/jsbi.1997.3845
press, 1992, Numerical Recipes in C, 547
10.1006/jsbi.1999.4174
hanszen, 1971, The optical transfer theory of the electron microscope: fundamental principles and applications, Advances in Optical and Electron Microscopy, 4, 1
10.1098/rstb.1971.0040