Identification of pyrethroid resistance associated mutations in the para sodium channel of the two‐spotted spider mite Tetranychus urticae (Acari: Tetranychidae)

Insect Molecular Biology - Tập 18 Số 5 - Trang 583-593 - 2009
Anastasia Tsagkarakou1, Thomas Van Leeuwen2, Jahangir Khajehali2, Aris Ilias3, Maria Grispou1, Martin S. Williamson4, Luc Tirry2, John Vontas5,3
1National Agricultural Research Foundation (N.AG.RE.F.), Laboratory of Entomology and Agricultural Zoology, Plant Protection Institute of Heraklion, Heraklion, Greece.
2Laboratory of Agrozoology Department of Crop Protection Faculty of Agricultural and Applied Biological Sciences, Ghent University, Ghent, Belgium
3Laboratory of Pesticide Science, Agricultural University of Athens, Athens, Greece
4Biological Chemistry Department, Rothamsted Research, Harpenden, UK; and
5Faculty of Biotechnology and Applied Biology, Department of Biology, University of Crete, Greece

Tóm tắt

AbstractWe investigated pyrethroid resistance mechanisms in Tetranychus urticae strains from Greece. Combined bioassay, biochemical and synergistic data indicated that although P450 mono‐oxygenase activities were associated with the trait, target site insensitivity was the major resistance component. A 3.3 kb cDNA fragment of the T. urticae para sodium channel gene encompassing segment 4 of domain II to segment 6 of domain IV was obtained by a degenerate PCR strategy. The T. urticae sequence showed highest identity (56%) to the scabies mite, Sarcoptes scabiei, and was phylogenetically classified within the divergent group of Arachnida. Comparison of resistant and susceptible strains identified the point mutation F1538I in segment 6 of domain III, which is known to confer strong resistance to pyrethroids, along with a second mutation (A1215D) in the intracellular linker connecting domains II and III with an unknown role. Three transcripts were identified corresponding to the k and l alternative exons. The mode of inheritance of resistance was confirmed as incompletely recessive, which is consistent with a target site mechanism for pyrethroids.

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Insect Molecular Biology

Tập 18 Số 5

583-593

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