Identification of prohibitin as a Chikungunya virus receptor protein

Journal of Medical Virology - Tập 84 Số 11 - Trang 1757-1770 - 2012
Phitchayapak Wintachai1, Nitwara Wikan1, Atichat Kuadkitkan1, Thitigun Jaimipuk1, Sukathida Ubol2,3, Rojjanaporn Pulmanausahakul1, Prasert Auewarakul2,4, Watchara Kasinrerk5, Wei Weng6, Mingkwan Greenwood1, Atchara Paemanee7, Suthathip Kittisenachai7, Sittiruk Roytrakul7, Stan B. Sidhu2,1
1Institute of Molecular Biosciences, Mahidol University, Nakorn Pathom, Thailand
2Center for Emerging and Neglected Infectious Diseases, Mahidol University, Nakorn Pathom, Thailand
3Faculty of Science, Department of Microbiology, Mahidol University, Nakorn Pathom, Thailand
4Department of Microbiology, Siriraj Hospital, Mahidol University, Nakorn Pathom, Thailand
5Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
6Mahidol University International College, Mahidol University, Nakorn Pathom, Thailand
7National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, PathumThani, Thailand

Tóm tắt

AbstractChikungunya virus (CHIKV) has recently re‐emerged causing millions of infections in countries around the Indian Ocean. While CHIKV has a broad host cell range and productively infects a number of different cell types, macrophages have been identified as a potential viral reservoir serving to increase the duration of symptoms. To date no CHIKV interacting protein has been characterized and this study sought to identify CHIKV binding proteins expressed on target cell membranes. Two‐dimensional virus overlay identified prohibitin (PHB) as a microglial cell expressed CHIKV binding protein. Co‐localization, co‐immunoprecipitation as well as antibody and siRNA mediated infection inhibition studies all confirmed a role for PHB in mediating internalization of CHIKV into microglial cells. PHB is the first identified CHIKV receptor protein, and this study is evidence that PHB may play a role in the internalization of multiple viruses. J. Med. Virol. 84:1757–1770, 2012. © 2012 Wiley Periodicals, Inc.

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