Identification of prohibitin as a Chikungunya virus receptor protein

Journal of Medical Virology - Tập 84 Số 11 - Trang 1757-1770 - 2012
Phitchayapak Wintachai1, Nitwara Wikan1, Atichat Kuadkitkan1, Thitigun Jaimipuk1, Sukathida Ubol2,3, Rojjanaporn Pulmanausahakul1, Prasert Auewarakul2,4, Watchara Kasinrerk5, Wei Weng6, Mingkwan Greenwood1, Atchara Paemanee7, Suthathip Kittisenachai7, Sittiruk Roytrakul7, Stan B. Sidhu2,1
1Institute of Molecular Biosciences, Mahidol University, Nakorn Pathom, Thailand
2Center for Emerging and Neglected Infectious Diseases, Mahidol University, Nakorn Pathom, Thailand
3Faculty of Science, Department of Microbiology, Mahidol University, Nakorn Pathom, Thailand
4Department of Microbiology, Siriraj Hospital, Mahidol University, Nakorn Pathom, Thailand
5Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand
6Mahidol University International College, Mahidol University, Nakorn Pathom, Thailand
7National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, PathumThani, Thailand

Tóm tắt

AbstractChikungunya virus (CHIKV) has recently re‐emerged causing millions of infections in countries around the Indian Ocean. While CHIKV has a broad host cell range and productively infects a number of different cell types, macrophages have been identified as a potential viral reservoir serving to increase the duration of symptoms. To date no CHIKV interacting protein has been characterized and this study sought to identify CHIKV binding proteins expressed on target cell membranes. Two‐dimensional virus overlay identified prohibitin (PHB) as a microglial cell expressed CHIKV binding protein. Co‐localization, co‐immunoprecipitation as well as antibody and siRNA mediated infection inhibition studies all confirmed a role for PHB in mediating internalization of CHIKV into microglial cells. PHB is the first identified CHIKV receptor protein, and this study is evidence that PHB may play a role in the internalization of multiple viruses. J. Med. Virol. 84:1757–1770, 2012. © 2012 Wiley Periodicals, Inc.

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Tài liệu tham khảo

10.1371/journal.pone.0034800

10.1099/vir.0.83357-0

10.1099/vir.0.82714-0

10.1074/jbc.M304877200

10.3201/eid1402.071024

10.1128/MCB.18.7.4043

10.1371/journal.pone.0011479

10.1086/517537

10.1016/0003-2697(76)90527-3

10.1016/j.virol.2007.09.028

10.1002/jmv.20786

10.4161/cbt.7.6.6281

10.1016/j.pneurobio.2009.12.006

10.1074/jbc.271.49.31679

10.1371/journal.pmed.0050060

10.1016/j.molcel.2004.05.002

10.1099/vir.0.015743-0

10.1210/me.2007-0400

10.4049/jimmunol.0904181

10.1016/0014-5793(94)01444-6

10.1586/eri.10.92

10.1016/0304-3940(94)11792-H

10.3201/eid1212.060710

10.1016/j.yexcr.2008.01.005

10.1038/nm1048

10.1242/dev.00160

10.1016/j.virol.2010.07.015

10.1016/j.virol.2005.03.015

10.1172/JCI40104

10.1086/529444

10.1016/j.bcmd.2011.06.005

10.1093/nar/gkn901

10.1074/mcp.M800250-MCP200

10.1016/j.bbamcr.2008.05.013

10.1101/gad.460708

10.1111/j.1582-4934.2006.tb00404.x

10.1016/j.virol.2010.12.061

10.1016/j.meegid.2010.07.012

10.3201/eid1508.081486

10.1093/emboj/19.11.2444

10.1007/s00018-002-8411-0

10.1016/j.imbio.2008.11.010

10.1007/s00705-010-0728-7

10.1016/j.cll.2009.10.003

10.1016/j.ijid.2011.06.002

10.1128/JVI.79.8.4557-4567.2005

10.1016/S0140-6736(07)61779-6

10.1016/j.molcel.2010.10.006

10.1016/0035-9203(55)90080-8

10.1017/S0022172400044442

10.1074/jbc.M311984200

10.1016/S0021-9258(18)37476-3

Sam IC, 2010, Chikungunya virus‐associated death in Malaysia, Trop Biomed, 27, 343

10.1016/j.virusres.2008.02.004

10.1371/journal.pmed.0030263

10.1038/nrmicro2368

10.1073/pnas.0407536101

10.1016/j.virol.2009.07.024

10.1371/journal.ppat.0030089

10.1016/j.bcmd.2011.11.004

10.1159/000105443

10.1186/1423-0127-16-17

10.1128/JVI.78.22.12647-12656.2004

10.1210/endo.142.9.8354

10.1371/journal.ppat.0030201

10.1371/journal.pone.0006835

Ubol S, 1991, Identification of a putative alphavirus receptor on mouse neural cells, J Virol, 65, 6913, 10.1128/jvi.65.12.6913-6921.1991

10.1371/journal.pone.0001168

10.1038/nature09555

10.1128/jvi.66.8.4992-5001.1992

10.1038/sj.onc.1205944

10.1371/journal.pone.0031102

10.3201/eid1210.060529

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Journal of Medical Virology

Tập 84 Số 11

1757-1770

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