Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera
Tóm tắt
Larval molting and metamorphosis are important physiological processes in the life cycle of the holometabolous insect. We used suppression subtractive hybridization (SSH) to identify genes differentially expressed during larval molting and metamorphosis. We performed SSH between tissues from a variety of developmental stages, including molting 5th and feeding 6th instar larvae, metamorphically committed and feeding 5th instar larvae, and feeding 5th instar and metamorphically committed larvae. One hundred expressed sequence tags (ESTs) were identified and included 73 putative genes with similarity to known genes, and 27 unknown ESTs. SSH results were further characterized by dot blot, Northern blot, and RT-PCR. The expression levels of eleven genes were found to change during larval molting or metamorphosis, suggesting a functional role during these processes. These results provide a new set of genes expressed specifically during larval molt or metamorphosis that are candidates for further studies into the regulatory mechanisms of those stage-specific genes during larval molt and metamorphosis
Tài liệu tham khảo
Mesce KA, Fahrbach SE: Integration of endocrine signals that regulate insect ecdysis. Front Neuroendocrinol. 2002, 23: 179-199.
Lee CY, Wendel DP, Reid P, Lam G, Thummel CS, Baehrecke EH: E93 directs steroid-triggered programmed cell death in Drosophila. Mol Cell. 2000, 6: 433-43.
Thummel CS: Steroid-triggered death by autophagy. BioEssays. 2001, 23: 677-682.
Riddiford LM, Hiruma K, Zhou X, Nelson CA: Insights into the molecular basis of the hormonal control of molting and metamorphosis from Manduca sexta and Drosophila melanogaster. Insect Biochem Mol Biol. 2003, 33: 1327-1338.
Riddiford LM, Cherbas P, Truman JW: Ecdysone receptors and their biological actions. Vitam Horm. 2000, 60: 1-73.
Frand AR, Russel S, Ruvkun G: Functional Genomic Analysis of C. elegans Molting. PLoS Biol. 2005, 1719-1733.
Yin VP, Thummel CS: Mechanisms of steroid-triggered programmed cell death in Drosophila. Semin Cell Dev Biol. 2005, 16: 237-243.
White KP, Rifkin SA, Hurban P, Hogness DS: Microarray analysis of Drosophila development during metamorphosis. Science. 1999, 286: 2179-2184.
Beckstead RB, Lam G, Thummel CS: The genomic response to 20-hydroxyecdysone at the onset of Drosophila metamorphosis. Genome Biol. 2005, 6: R99-
Li TR, White KP: Tissue-specific gene expression and ecdysone-regulated genomic networks in Drosophila. Dev Cell. 2003, 5: 59-72.
Arbeitman MN, Furlong EE, Imam F, Johnson E, Null BH, Baker BS, Krasnow MA, Scott MP: Gene expression during the life cycle of Drosophila melanogaster. Science. 2002, 297: 2270-2275.
Zhao X-F, Wang J-X, Xu X-L, Li Z-M, Kang C-J: Molecular cloning and expression patterns of molt-regulating transcription factor-hhr3 from Helicoverpa armigera. Insect Mol Biol. 2004, 13: 407-412.
Zhao X-F, He H-J, Dong D-J, Wang J-X: Identification of Differentially Expressed Proteins during Larval Molting of Helicoverpa armigera. J Proteome Res. 2006, 5: 164-169.
Diatchenko L, Lau Y-FC, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD: Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA. 1996, 93: 6025-6030.
Gurskaya NG, Diatchenko L, Chenchik A, Siebert PD, Khaspekov GL, Lukyanov KA, Vagner LL, Ermolaeva OD, Lukyanov SA, Sverdlov ED: Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: Cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristate 13-acetate. Anal Biochem. 1996, 240: 90-97.
Ote M, Mita K, Kawasaki H, Daimon T, Kobayashi M, Shimada T: Identification of molting fluid carboxypeptidase A (MF-CPA) in Bombyx mori. Comp Biochem Physiol B Biochem Mol Biol. 2005, 141: 314-322.
Guiliano DB, Hong X, McKerrow JH, Blaxter ML, Oksov Y, Liu J, Ghedin E, Lustigman S: A gene family of cathepsin L-like proteases of filarial nematodes are associated with larval molting and cuticle and eggshell remodeling. Mol Biochem Parasitol. 2004, 136: 227-242.
Bhattacharya A, Steward R: The Drosophila homolog of NTF-2, the nuclear transport factor-2, is essential for immune response. EMBO Rep. 2002, 3: 378-383.
Hewes RS, Schaefer AM, Taghert PH: The cryptocephal gene (ATF4) encodes multiple basic-leucine zipper proteins controlling molting and metamorphosis in Drosophila. Genetics. 2000, 155: 1711-1723.
Jones G, O'Mahony P, Chang S, Schachtschabel U: Identification of regulatory sequences of juvenile hormone-sensitive and -insensitive serum protein-encoding genes. Gene. 1996, 173: 209-214.
Purdy A, Uyetake L, Cordeiro MG, Su TT: Regulation of mitosis in response to damaged or incompletely replicated DNA require different levels of Grapes (Drosophila Chk1). J Cell Sci. 2005, 118: 3305-3315.
Costanzi J, Sidransky D, Navon A, Goldsweig H: Ribonucleases as a novel pro-apoptotic anticancer strategy: review of the preclinical and clinical data for ranpirnase. Cancer Invest. 2005, 23: 643-650.
Kasai H, Nadano D, Hidaka E, Higuchi K, Kawakubo M, Sato TA, Nakayama J: Differential expression of ribosomal proteins in human normal and neoplastic colorectum. J Histochem Cytochem. 2003, 51: 567-574.
Gray JP, Davis JW, Gopinathan L, Leas TL, Nugent CA, Vanden Heuvel JP: The ribosomal protein rpL11 associates with and inhibits the transcriptional activity of peroxisome proliferator-activated receptor-alpha. Toxicol Sci. 2006, 89: 535-546.
Dai MS, Zeng SX, Jin Y, Sun XX, David L, Lu H: Ribosomal protein L23 activates p53 by inhibiting MDM2 function in response to ribosomal perturbation but not to translation inhibition. Mol Cell Biol. 2004, 24: 7654-7668.
Jin A, Itahana K, O'Keefe K, Zhang Y: Inhibition of HDM2 and activation of p53 by ribosomal protein L23. Mol Cell Biol. 2004, 24: 7669-7680.
Yoo YA, Kim MJ, Park JK, Chung YM, Lee JH, Chi SG, Kim JS, Yoo YD: Mitochondrial ribosomal protein L41 suppresses cell growth in association with p53 and p27Kip1. Mol Cell Biol. 2005, 25: 6603-6616.
Kim MJ, Yoo YA, Kim HJ, Kang S, Kim YG, Kim JS, Yoo YD: Mitochondrial ribosomal protein L41 mediates serum starvation-induced cell-cycle arrest through an increase of p21(WAF1/CIP1). Biochem Biophys Res Commun. 2005, 338: 1179-1184.
Khanna N, Sen S, Sharma H, Singh N: S29 ribosomal protein induces apoptosis in H520 cells and sensitizes them to chemotherapy. Biochem Biophys Res Commun. 2003, 304 (1): 26-35.
Khanna N, Reddy VG, Tuteja N, Singh N: Differential gene expression in apoptosis: identification of ribosomal protein S29 as an apoptotic inducer. Biochem Biophys Res Commun. 2000, 277: 476-486.
Squires CL, Zaporojets D: Proteins shared by the transcription and translation machines. Annu Rev Microbiol. 2000, 54: 775-798.
Russell VW, Dunn PE: Lysozyme in the midgut of Manduca sexta during metamorphosis. Arch Insect Biochem Physiol. 1991, 17: 67-80.
Inohara N, Nunez G: ML – a conserved domain involved in innate immunity and lipid metabolism. Trends Biochem Sci. 2002, 27: 219-21.
Mahoney JA, Ntolosi B, DaSilva RP, Gordon S, McKnight AJ: Cloning and characterization of CPVL, a novel serine carboxypeptidase, from human macrophages. Genomics. 2001, 72: 243-251.
Harris J, Schwinn N, Mahoney JA, Lin HH, Shaw M, Howard CJ, da Silva RP, Gordon S: A vitellogenic-like carboxypeptidase expressed by human macrophages is localized in endoplasmic reticulum and membrane ruffles. Int J Exp Pathol. 2006, 87: 29-39.
Liu J, Shi G-P, Zhang W-Q, Zhang G-R, Xu W-H: Cathepsin L function in insect moulting: molecular cloning and functional analysis in cotton bollworm, Helicoverpa armigera. Insect Mo Biol. 2006, 15 (6): 823-834.
BLASTX. [http://www.ncbi.nlm.nih.gov.]