Identification of functional type 1 ryanodine receptors in mouse dendritic cells

FEBS Letters - Tập 512 - Trang 67-70 - 2002
Peta J. O’Connell1, Vitaly A. Klyachko2, Gerard P. Ahern3
1The John P. Robarts Research Institute, London, ON, Canada N6A 5K8
2Department of Physiology and Biophysics PhD Program, University of Wisconsin-Madison, Madison, WI 53706, USA
3Department of Pharmacology, Georgetown University, Washington DC, 20007, USA

Tóm tắt

Ca2+ signaling plays an important role in the function of dendritic cells (DC), the specialized antigen‐presenting cells of the immune system. Here we describe functional ryanodine receptor (RyR) Ca2+ release channels in murine, bone marrow‐derived DC. RT‐PCR analysis identified selective expression of the type 1 RyR, with higher levels detected in immature rather than mature DC. The RyR activators caffeine, FK506, ryanodine and 4‐chloro‐m‐cresol mobilized Ca2+ in DC, and responses to 4‐chloro‐m‐cresol were inhibited by dantrolene. Furthermore, activation of RyRs both inhibited subsequent inositol trisphosphate‐mediated Ca2+ release and provoked store‐operated Ca2+ entry, suggesting a functional interaction between these intracellular Ca2+ channels. Thus, the RyR1 channel may play an intrinsic role in Ca2+ signaling in DC.

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FEBS Letters

Tập 512

67-70

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