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Xác định các gen tiềm năng trong lúa đối với bệnh chết chóc bằng cách giải trình tự toàn bộ bộ gen
Tóm tắt
Những tiến bộ gần đây trong việc giải trình tự toàn bộ bộ gen đã cho phép xác định các gen có liên quan đến độ nhạy cảm với bệnh ở người. Mục tiêu của nghiên cứu của chúng tôi là khai thác các bộ gen toàn bộ của 13 dòng lúa thuần chủng (Oryza sativa L.) để xác định các SNP không đồng nghĩa (nsSNPs) và các gen tiềm năng cho khả năng kháng bệnh bạc lá, một bệnh có ý nghĩa toàn cầu. Giải trình tự toàn bộ bộ gen bằng nền tảng Illumina GA IIx cho độ phủ trung bình 5× với ~700 K biến thể được phát hiện trên mỗi dòng so với bộ gen tham chiếu Nipponbare. Hai chiến lược lọc đã được phát triển để xác định nsSNPs giữa hai nhóm dòng đã biết có khả năng kháng và nhạy cảm. Tổng cộng có 333 nsSNPs được phát hiện trong các dòng kháng, không có trong nhóm nhạy cảm. Các biến thể được chọn có liên quan đến kháng đã được tìm thấy trên 11 trong số 12 nhiễm sắc thể. Hơn 200 gen với nsSNPs đã chọn được phân loại vào 42 thể loại dựa trên họ gen/định nghĩa gen. Một số gen tiềm năng thuộc các họ đã được báo cáo trong các nghiên cứu trước đây, và ba vùng mới với các ứng viên chưa từng thấy cũng đã được xác định. Một tập hợp con gồm 24 nsSNPs được phát hiện trong 23 gen đã được chọn để nghiên cứu thêm. Các alen cá nhân của 24 nsSNPs được đánh giá qua PCR mà sự hiện diện hoặc vắng mặt của chúng tương ứng với các kiểu hình kháng hoặc nhạy cảm đã biết của chín dòng bổ sung. Giải trình tự Sanger đã xác nhận sự hiện diện của 12 nsSNPs đã chọn trong hai dòng. Các alen nsSNP "kháng" đã được phát hiện trong hai dòng O. nivara, điều này gợi ý rằng nguồn kháng có thể xảy ra trong các loài Oryza khác. Kết quả từ nghiên cứu này cung cấp nền tảng cho các nghiên cứu cơ bản trong tương lai và việc chọn giống giúp hỗ trợ khả năng kháng bệnh bạc lá trên lúa.
Từ khóa
#gen tiềm năng #lúa #bệnh bạc lá #SNP không đồng nghĩa #giải trình tự bộ genTài liệu tham khảo
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