Identification of a <i>Hevea brasiliensis</i> Latex Manganese Superoxide Dismutase (Hev b 10) as a Cross-Reactive Allergen

International Archives of Allergy and Immunology - Tập 125 Số 2 - Trang 120-127 - 2001
Stefan Wagner1, Slawomir Sowka1, Christine Mayer2, Reto Crameri2, M. Focke1, Viswanath P. Kurup3,4, Otto Scheiner1, Heimo Breiteneder1
1Department of Pathophysiology, University of Vienna, Vienna, Austria
2Swiss Institute of Allergy and Asthma Research, Davos, Switzerland
3Allergy Immunology Division, Medical College of Wisconsin, Milwaukee, Wisc.,
4Research Service, V.A. Medical Center, Milwaukee, Wisc., USA

Tóm tắt

<i>Background:</i> Cross-reactive allergens play an increasingly important role in latex allergy in complicating both the diagnosis and time course of allergic symptoms. Manganese superoxide dismutase (MnSOD), a ubiquitous protein of prokaryotic and eukaryotic organisms, was described as a cross-reactive allergen in <i>Aspergillus fumigatus</i>. Little information is available on the importance of this pan-allergen in <i>Hevea brasiliensis</i> latex. The aim of this study was to clone and express MnSOD from <i>H. brasiliensis</i> latex, and to obtain the soluble and immunologically active recombinant allergen for diagnosis of latex allergy and to investigate possible cross-reactivities with the structurally related <i>A. fumigatus</i> and human MnSODs. <i>Methods:</i> A complementary DNA coding for <i>Hevea</i> latex MnSOD was amplified by PCR. The recombinant protein was produced in <i>Escherichia coli</i> with an N-terminal hexahistidyl tag. Enzymatic activity of the recombinant protein was determined using an enzyme assay for SODs. IgE immunoblotting and IgE inhibition assays were performed to characterize the recombinant allergen and its cross-reactivity. <i>Results:</i> A <i>Hevea</i> latex MnSOD consisting of 206 amino acid residues was cloned and expressed in <i>E. coli</i>. The allergen was designated Hev b 10. The recombinant protein was enzymatically active, indicating the correct folding of the protein. In immunoblots, latex- as well as <i>A. fumigatus</i>-allergic patients revealed IgE binding to recombinant (r)Hev b 10. Cross-reactivity to Asp f 6, the MnSOD from <i>A. fumigatus</i>, and human MnSOD was determined by inhibition of IgE binding to these MnSODs by rHev b 10. <i>Conclusions:</i> Hev b 10 is a new cross-reactive allergen of <i>H. brasiliensis</i> which belongs to the ‘latex-mold’ group of latex allergens. Furthermore, it is a candidate for primary sensitization in patients allergic to the pan-allergen MnSOD.

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Thông tin xuất bản

International Archives of Allergy and Immunology

Tập 125 Số 2

120-127

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