Identification of Potentially DiagnosticLeishmania braziliensisAntigens in Human Cutaneous Leishmaniasis by Immunoblot Analysis

American Society for Microbiology - Tập 7 Số 2 - Trang 318-321 - 2000
Maria Edileuza Felinto de Brito1, Mitzi G. Mendonça2, Yara M. Gomes1, Márcio Lobo Jardim2, Frederico G. C. Abath1
1Departmento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz,1 and
2Departmento de Dermatologia, Universidade Federal de Pernambuco,2 Cidade Universitária, 50670-420 Recife, Brazil

Tóm tắt

ABSTRACT

The antibody response in patients with American cutaneous leishmaniasis was analyzed by immunoblotting with soluble and insoluble antigens ofLeishmania braziliensis. The recognition of the 27- and/or 30-kDa soluble antigens was considered relevant for the diagnosis of cutaneous leishmaniasis. Immunoblotting was found to be significantly more sensitive and specific than indirect immunofluorescence and enzyme-linked immunosorbent assay.

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