Hydropriming and Biopriming Improve Medicago truncatula Seed Germination and Upregulate DNA Repair and Antioxidant Genes

Genes - Tập 11 Số 3 - Trang 242
Chiara Forti1, Ajay Shankar2, Anjali Singh2, Alma Balestrazzi1, Vishal Prasad2, Anca Macovei1
1Department of Biology and Biotechnology ‘L. Spallanzani’, University of Pavia, Via Ferrata 9, 27100 Pavia Italy
2Institute of Environment and Sustainable Development, Banaras Hindu University, 221005 Varanasi, India

Tóm tắt

Seed germination is a critical parameter for the successful development of sustainable agricultural practices. While seed germination is impaired by environmental constraints emerging from the climate change scenario, several types of simple procedures, known as priming, can be used to enhance it. Seed priming is defined as the process of regulating seed germination by managing a series of parameters during the initial stages of germination. Hydropriming is a highly accessible and economic technique that involves soaking of seeds in water followed by drying. Biopriming refers to the inoculation of seeds with beneficial microorganism. The present study aims to investigate whether hydropriming and biopriming could enhance seed germination. Thereby, the germination of Medicago truncatula seeds exposed to hydropriming and/or Bacillus spp. isolates was monitored for two-weeks. The seeds were sown in trays containing two types of in situ agricultural soils collected from Northern India (Karsara, Varanasi). This region is believed to be contaminated by solid waste from a nearby power plant. Phenotypic parameters had been monitored and compared to find the most appropriate combination of treatments. Additionally, qRT-PCR was used to evaluate the expression levels of specific genes used as molecular indicators of seed quality. The results show that, while hydropriming significantly enhanced seed germination percentage, biopriming resulted in improved seedling development, represented by increased biomass rather than seedling length. At a molecular level, this is reflected by the upregulation of genes involved in DNA damage repair and antioxidant defence. In conclusion, hydropriming and biopriming are efficient to improve seed germination and seedling establishment in soils collected from damaged sites of Northern India; this is reflected by morphological parameters and molecular hallmarks of seed quality.

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Tài liệu tham khảo

ISTA (International Seed Testing Association) (2018). International Rules for Seed Testing, ISTA.

Paparella, 2015, Seed priming: State of the art and new perspectives, Plant Cell Rep., 34, 1281, 10.1007/s00299-015-1784-y

Macovei, 2017, Systems biology and genome-wide approaches to unveil the molecular players involved in the pre-germinative metabolism: Implications on seed technology traits, Plant Cell Rep., 36, 669, 10.1007/s00299-016-2060-5

Ashraf, 1993, DNA synthesis in osmoprimed leek (Allium porrum L.) seeds and evidence for repair and replication, Seed Sci. Res., 3, 15, 10.1017/S0960258500001525

Balestrazzi, 2011, Seed imbibition in Medicago truncatula Gaertn: Expression profiles of DNA repair genes in relation to PEG-mediated stress, J. Plant Physiol., 168, 706, 10.1016/j.jplph.2010.10.008

Pagano, 2017, The seed repair response during germination: Disclosing correlations between DNA repair, antioxidant response, and chromatin remodeling in Medicago truncatula, Front. Plant Sci., 8, 1972, 10.3389/fpls.2017.01972

Pagano, 2019, Metabolic and gene expression hallmarks of seed germination uncovered by sodium butyrate in Medicago truncatula, Plant Cell Environ., 42, 259, 10.1111/pce.13342

Pagano, 2019, Metabolic signatures of germination triggered by kinetin in Medicago truncatula, Sci. Rep., 9, 10466, 10.1038/s41598-019-46866-6

Waterworth, 2019, Seeds and the art of genome maintenance, Front. Plant Sci., 10, 706, 10.3389/fpls.2019.00706

Paparella, 2016, Physical methods for seed invigoration: Advantages and challenges in seed technology, Front. Plant Sci., 7, 646

Casenave, 2007, Hydropriming as a pre-treatment for cotton germination under thermal and water stress conditions, Seed Sci. Technol., 35, 88, 10.15258/sst.2007.35.1.08

Yan, 2016, Hydro-priming increases seed germination and early seedling growth in two cultivars of Napa cabbage (Brassica rapa subsp. pekinensis) grown under salt stress, J. Hortic. Sci. Biotech., 91, 421, 10.1080/14620316.2016.1162031

Karalija, 2018, The effect of hydro and proline seed priming on growth, proline and sugar content, and antioxidant activity of maize under cadmium stress, Environ. Sci. Pollut. Res. Int., 25, 33370, 10.1007/s11356-018-3220-7

Płażek, A., Dubert, F., Kopeć, P., Dziurka, M., Kalandyk, A., Pastuszak, J., and Wolko, B. (2018). Seed hydropriming and smoke water significantly improve low-temperature germination of Lupinus angustifolius L.. Int. J. Mol. Sci., 19.

Płażek, A., Dubert, F., Kopeć, P., Dziurka, M., Kalandyk, A., Pastuszak, J., Waligórski, P., and Wolko, B. (2018). Long-term effects of cold on growth, development and yield of narrow-Leaf lupine may be alleviated by seed hydropriming or butenolide. Int. J. Mol. Sci., 19.

Biswas, 2000, Rhizobia inoculation improves nutrient uptake and growth of lowland rice, Soil Sci. Soc. Am. J., 64, 1644, 10.2136/sssaj2000.6451644x

Yadav, 2018, Seed bio-priming of baby corn emerged as a viable strategy for reducing mineral fertilizer use and increasing productivity, Sci. Hort., 241, 93, 10.1016/j.scienta.2018.06.096

Rozier, 2019, Biopriming of maize germination by the plant growth-promoting rhizobacterium Azospirillum lipoferum CRT1, J. Plant Physiol., 237, 111, 10.1016/j.jplph.2019.04.011

Macovei, 2014, Synergistic exposure of rice seeds to different doses of γ-ray and salinity stress resulted in increased antioxidant enzyme activities and gene-specific modulation of TC-NER pathway, Biomed. Res. Int., 2014, 676934, 10.1155/2014/676934

Ibrahim, 2016, Seed priming to alleviate salinity stress in germinating seeds, J. Plant Physiol., 192, 38, 10.1016/j.jplph.2015.12.011

Li, 2017, The synergistic priming effect of exogenous salicylic acid and H2O2 on chilling tolerance enhancement during maize (Zea mays L.) seed germination, Front. Plant Sci., 8, 1153, 10.3389/fpls.2017.01153

Bajwa, 2018, Seed priming with sorghum extracts and benzyl aminopurine improves the tolerance against salt stress in wheat (Triticum aestivum L.), Physiol. Mol. Biol. Plants, 24, 239, 10.1007/s12298-018-0512-9

FAO—Food and Agriculture Organization of the United States (2015). Status of the World’s Soil Resources Report (SWSR), FAO. Available online: http://www.fao.org/3/a-i5199e.pdf.

Machender, 2013, Assessment of trace element contamination in soils around Chinnaeru River Basin, Nalgonda District, India, Environ. Earth Sci., 70, 1021, 10.1007/s12665-012-2192-z

Paul, 2015, Spatial distribution and the extent of heavy metal and hexavalent chromium pollution in agricultural soils from Jajmau, India, Environ. Earth Sci., 73, 3565, 10.1007/s12665-014-3642-6

Kumar, 2019, Pollution assessment of heavy metals in soils of India and ecological risk assessment: A state-of-the-art, Chemosphere, 216, 449, 10.1016/j.chemosphere.2018.10.066

Gill, 2012, Cadmium at high dose perturbs growth, photosynthesis and nitrogen metabolism while at low dose it up regulates sulfur assimilation and antioxidant machinery in garden cress (Lepidium sativum L.), Plant Sci., 182, 112, 10.1016/j.plantsci.2011.04.018

Gill, 2013, Importance of nitric oxide in cadmium stress tolerance in crop plants, Plant Physiol. Biochem., 63, 254, 10.1016/j.plaphy.2012.12.001

Macovei, 2011, New insights on the barrel medic MtOGG1 and MtFPG functions in relation to oxidative stress response in planta and during seed imbibition, Plant Physiol. Biochem., 49, 1040, 10.1016/j.plaphy.2011.05.007

Edvan, R.L., and Santos, E.M. (2018). Tropical Forage Legumes in India: Status and Scope for Sustaining Livestock Production, Forage Groups, IntechOpen. Available online: https://www.intechopen.com/books/forage-groups/tropical-forage-legumes-in-india-status-and-scope-for-sustaining-livestock-production.

Farooq, 2018, Impact of abiotic stresses on grain composition and quality in food legumes, J. Agric. Food Chem., 66, 8887, 10.1021/acs.jafc.8b02924

Mestdagh, 2009, A novel and universal method for microRNA RT-qPCR data normalization, Genome Biol., 10, R64, 10.1186/gb-2009-10-6-r64

Untergrasser, 2012, Primer3--new capabilities and interfaces, Nucleic Acids Res., 40, e115, 10.1093/nar/gks596

Pfaffl, 2001, A new mathematical model for relative quantification in real-time RT-PCR, Nucleic Acids Res., 29, e45, 10.1093/nar/29.9.e45

Assaad, 2015, Rapid publication-ready MS-Word tables for two-way ANOVA, Springerplus, 4, 33, 10.1186/s40064-015-0795-z

Metsalu, 2015, Clustvis: A web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap, Nucleic Acids Res., 43, W566, 10.1093/nar/gkv468

Garcia, J., Barker, D.G., and Journet, E.-P. (2006). Seed storage and germination. Medicago Truncatula Handbook, CABI Press.

Baskin, 2004, A classification system for seed dormancy, Seed Sci. Res., 14, 1, 10.1079/SSR2003150

Gallardo, G., Lesignor, C., Darmency, M., Burstin, J., Thompson, R., Rochat, C., Boutin, J.-P., Kuester, H., Buitink, J., and Leprince, O. (2006). Seed biology of Medicago truncatula. The Medicago Truncatula Handbook, CABI Press. Available online: http://www.noble.org/MedicagoHandbook/.

Bolingue, 2010, Characterization of dormancy behaviour in seeds of the model legume Medicago truncatula, Seed Sci. Res., 20, 97, 10.1017/S0960258510000061

Balestrazzi, 2011, Unraveling the response of plant cells to cytotoxic saponins: Role of metallothionein and nitric oxide, Plant Signal Behav., 6, 516, 10.4161/psb.6.4.14746

Bhattacharyya, 2015, Soil degradation in India: Challenges and potential solutions, Sustainability, 7, 3528, 10.3390/su7043528

Heil, K., and Schmidhalter, U. (2017). The application of EM38: Determination of soil parameters, selection of soil sampling points and use in agriculture and archaeology. Sensors (Basel), 17.

Corwin, 2003, Application of soil electrical conductivity to precision agriculture: Theory, principles, and guidelines, Agron. J., 95, 455

Guretzky, 2004, Distribution of legumes along gradients of slope and soil electrical conductivity in pastures, Agron. Hortic. Fac. Publ., 96, 547

Cornell University Cooperative Extension (2020, February 25). Cornell University Cooperative Extension. Agronomy Fact Sheet Series (Fact Sheet 5): Soil pH for Field Crops. Available online: http://cceonondaga.org/resources/soil-ph-for-field-crops.

Neina, 2019, The role of soil pH in plant nutrition and soil remediation, Appl. Environ. Soil Sci., 5794869, 1, 10.1155/2019/5794869

Shukla, P. (2016). Rhizobacteria: Tools for the management of plant abiotic stresses. Microbial Biotechnology, CRC Press. [1st ed.].

Prasad, 2018, Improving phosphorus fertility in soil through microbial mediators, Int. J. Plant Environ., 4, 2, 10.18811/ijpen.v4i02.9

Song, 2017, Seed defense biopriming with bacterial cyclodipeptides triggers immunity in cucumber and pepper, Sci. Rep., 7, 14209, 10.1038/s41598-017-14155-9

Lechowska, K., Kubala, S., Wojtyla, Ł., Nowaczyk, G., Quinet, M., Lutts, S., and Garnczarska, M. (2019). New insight on water status in germinating Brassica napus seeds in relation to priming-improved germination. Int. J. Mol. Sci., 20.

Alscher, 2002, Role of superoxide dismutases (SODs) in controlling oxidative stress in plants, J. Exp. Bot., 53, 1331, 10.1093/jexbot/53.372.1331

Caverzan, 2012, Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection, Genet Mol. Biol., 35, 1011, 10.1590/S1415-47572012000600016

Nouman, 2014, Seed priming improves the emergence potential, growth and antioxidant system of Moringa oleifera under saline conditions, Plant Growth Regul., 73, 267, 10.1007/s10725-014-9887-y

Jisha, 2016, Seed priming with BABA (β-amino butyric acid): A cost-effective method of abiotic stress tolerance in Vigna radiata (L.) Wilczek, Protoplasma, 253, 277, 10.1007/s00709-015-0804-7

Islam, 2015, Priming-induced antioxidative responses in two wheat cultivars under saline stress, Acta Physiol Plant., 37, 153, 10.1007/s11738-015-1897-5

Zhang, F., Yu, J., Johnston, C.R., Wang, Y., Zhu, K., Lu, F., Zhang, Z., and Zou, J. (2015). Seed priming with polyethylene glycol induces physiological changes in sorghum (Sorghum bicolor L. Moench) seedlings under suboptimal soil moisture environments. PLoS ONE, 10.

Salah, 2015, Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress, Sci. Rep., 5, 14278, 10.1038/srep14278

Kubala, 2015, Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach, Plant Sci., 231, 94, 10.1016/j.plantsci.2014.11.008

Cobbet, 2002, Phytochelatins and metallothioneins: Roles in heavy metal detoxification and homeostasis, Annu. Rev. Plant Biol., 53, 159, 10.1146/annurev.arplant.53.100301.135154

Balestrazzi, 2011, Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba L.) suspension cultures exposed to alfalfa saponins, Plant Physiol., 141, 227, 10.1111/j.1399-3054.2010.01436.x

Balestrazzi, 2009, Expression of the PsMTA1 gene in white poplar engineered with the MAT system is associated to heavy metal tolerance and protection against 8-hydroxy-2′-deoxyguanosine mediated-DNA damage, Plant Cell Rep., 28, 1179, 10.1007/s00299-009-0719-x

Ventura, 2013, γ irradiation with different dose rates induces different DNA damage responses in Petunia x hybrida cells, J. Plant Physiol., 170, 780, 10.1016/j.jplph.2013.01.010

Mondoni, 2011, Seeds of alpine plants are short-lived: Implications for long-term conservation, Ann. Bot., 107, 171, 10.1093/aob/mcq222

Balestrazzi, 2013, DNA profiling, telomere analysis and antioxidant properties as tools for monitoring ex situ seed longevity, Ann. Bot., 111, 987, 10.1093/aob/mct058