Tế bào xương người in vitro

Calcified Tissue International - Tập 37 - Trang 453-460 - 1985
Pamela Gehron Robey1, John D. Termine1
1Mineralized Tissue Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, USA

Tóm tắt

Các nuôi cấy tế bào xương người đã được thiết lập bằng cách duy trì các mảnh xương được điều trị bằng collagenase trong môi trường có nồng độ Ca++ thấp. Các nuôi cấy tế bào thu được thể hiện hoạt tính phosphatase kiềm ở mức cao và tạo ra sự gia tăng đáng kể trong cAMP nội bào khi tiếp xúc với đoạn 1–34 của hormone cận giáp người. Với việc nuôi cấy liên tục, các tế bào tạo thành một ma trận ngoại bào dày, được khoáng hóa khi các nuôi cấy được cung cấp hàng ngày với nồng độ canxi bình thường, axit ascorbic tươi (50 μg/ml) và 10 mM β-glycerol phosphate. Về mặt sinh học tổng hợp, các tế bào này sản xuất collagen loại I (không có bất kỳ loại collagen loại III nào) và protein đặc hiệu cho xương, osteonectin. Ngoài ra, các tế bào này cũng sản xuất các đại phân tử sulfat hóa có điện di tương đồng với các proteoglycan xương đã được xác định dương tính trong các nuôi cấy song song của tế bào xương thai bò. Kỹ thuật này cung cấp một hệ thống hữu ích cho việc nghiên cứu chuyển hóa của osteoblast in vitro.

Từ khóa

#xương người #tế bào xương #nuôi cấy tế bào #osteoblast #phosphatase kiềm #collagen loại I #osteonectin #proteoglycan xương

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Thông tin
Thông tin xuất bản

Calcified Tissue International

Tập 37

453-460

Thông tin tác giả