Human blood monocytes are able to form extracellular traps

Journal of Leukocyte Biology - Tập 102 Số 3 - Trang 775-781 - 2017
Vanessa Granger1,2, Dorothée Faille3, Vanessa Marani2, Benoît Noël2, Yann Gallais2, Natacha Szely2, Héloïse Flament1, Marc Pallardy2, Sylvie Chollet‐Martin1,2, Luc de Chaisemartin1,2
1Immunology Department, Assistance Publique Hopitaux de Paris, Bichat Hospital , Paris, France
2Unité mixte de Recherche 996-Inflammation, Chemokines and Immunopathology, Institut National de la Santé et de la Recherche Médicale, Université Paris Sud, Université Paris-Saclay , Châtenay-Malabry, France
3Hematology Department, Assistance Publique Hopitaux de Paris, Bichat Hospital , Paris, France

Tóm tắt

Abstract Neutrophil extracellular traps (NETs) are extracellular DNA filaments formed during neutrophil activation. This process, called netosis, was originally associated with neutrophil antibacterial properties. However, several lines of evidence now suggest a major role for netosis in thrombosis, autoimmune diseases, and cancer. We demonstrate here that highly purified human blood monocytes are also capable of extracellular trap (ET) release in response to several stimuli. Monocyte ETs display a morphology analogous to NETs and are associated with myeloperoxidase (MPO), lactoferrin (LF), citrullinated histones, and elastase. Monocyte ET release depends on oxidative burst but not on MPO activity, in contrast to neutrophils. Moreover, we demonstrate procoagulant activity for monocyte ETs, a feature that could be relevant to monocyte thrombogenic properties. This new cellular mechanism is likely to have implications in the multiple pathologic contexts where monocytes are implicated, such as inflammatory disorders, infection, or thrombosis.

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