Human adult neurogenesis across the ages: An immunohistochemical study

Neuropathology and Applied Neurobiology - Tập 42 Số 7 - Trang 621-638 - 2016
Claude Dennis1, Lisa S. Suh2,1, Michael Rodriguez1, Jillian J. Kril1, Greg T. Sutherland1
1Discipline of Pathology Sydney Medical School University of Sydney Camperdown NSW Australia
2Dementia Research Unit School of Medical Sciences University of New South Wales Kensington NSW Australia

Tóm tắt

AbstractAims

Neurogenesis in the postnatal human brain occurs in two neurogenic niches; the subventricular zone (SVZ) in the wall of the lateral ventricles and the subgranular zone (SGZ) of the hippocampus. The extent to which this physiological process continues into adulthood is an area of ongoing research. This study aimed to characterize markers of cell proliferation and assess the efficacy of antibodies used to identify neurogenesis in both neurogenic niches of the human brain.

Methods

Cell proliferation and neurogenesis were simultaneously examined in the SVZ and SGZ of 23 individuals aged 0.2–59 years, using immunohistochemistry and immunofluorescence in combination with unbiased stereology.

Results

There was a marked decline in proliferating cells in both neurogenic niches in early infancy with levels reaching those seen in the adjacent parenchyma by 4 and 1 year of age, in the SVZ and SGZ, respectively. Furthermore, the phenotype of these proliferating cells in both niches changed with age. In infants, proliferating cells co‐expressed neural progenitor (epidermal growth factor receptor), immature neuronal (doublecortin and beta III tubulin) and oligodendrocytic (Olig2) markers. However, after 3 years of age, microglia were the only proliferating cells found in either niche or in the adjacent parenchyma.

Conclusions

This study demonstrates a marked decline in neurogenesis in both neurogenic niches in early childhood, and that the sparse proliferating cells in the adult brain are largely microglia.

Từ khóa


Tài liệu tham khảo

10.1080/07853890500371890

10.3389/fncel.2013.00005

10.3389/fncel.2015.00365

10.1111/j.1749-6632.2009.03913.x

10.1038/nn.2298

10.1038/4151030a

10.1126/science.1248903

10.1126/science.1136281

10.1111/j.1460-9568.2004.03813.x

10.1038/nature10487

10.1371/journal.pone.0008809

10.1016/j.cell.2013.05.002

10.1016/j.cell.2014.01.044

10.1016/j.neuron.2012.03.030

10.1369/jhc.7A7187.2007

10.1016/j.expneurol.2013.03.020

10.1002/cne.20798

10.1016/S0896-6273(02)01133-9

10.1523/JNEUROSCI.14-09-05399.1994

10.1016/0165-3806(84)90097-X

10.1016/j.pneurobio.2011.01.005

10.1016/S0169-328X(98)00040-0

10.1007/s11011-013-9469-0

10.1523/JNEUROSCI.1924-05.2005

10.1523/JNEUROSCI.1299-06.2006

10.1038/cr.2011.83

10.1073/pnas.1532244100

10.1016/j.neuroscience.2004.12.051

10.1093/brain/awr256

10.1177/44.11.8918901

10.1016/S0169-328X(00)00092-9

10.1042/BST0370605

10.1038/srep18779

10.1093/brain/awm264

10.1371/journal.pone.0025760

10.1111/ejn.12269

10.1016/j.nbd.2006.04.017

10.1016/j.neuron.2011.05.004

10.1073/pnas.1017099108

10.3389/fncel.2013.00258

10.1002/glia.20243

10.1111/j.1474-9726.2010.00556.x

10.1523/JNEUROSCI.3454-14.2015

10.1371/journal.pone.0036016

10.1523/JNEUROSCI.3713-08.2008

10.1523/JNEUROSCI.22-02-00437.2002

10.1038/npp.2013.5

10.1046/j.1460-9568.2003.02647.x

10.1002/cne.1040

10.1073/pnas.94.19.10409

10.1111/j.1460-9568.2009.07061.x

10.1073/pnas.96.10.5768

10.1016/j.neurobiolaging.2011.03.008

10.1038/nn1928

10.1016/j.neuroscience.2013.04.006

10.1126/science.1065467

10.1002/hipo.22401

10.1073/pnas.0605177103

10.1111/j.1750-3639.2009.00295.x

Doorn KJ, 2014, Hippocampal proliferation is increased in presymptomatic Parkinson's disease and due to microglia, Neural Plast, 2014, 959154, 10.1155/2014/959154

10.1111/j.1460-9568.2008.06518.x

Thông tin
Thông tin xuất bản

Neuropathology and Applied Neurobiology

Tập 42 Số 7

621-638

Thông tin tác giả