Homologs of Caenorhabditis elegans Chemosensory Genes Have Roles in Behavior and Chemotaxis in the Root-Knot Nematode Meloidogyne incognita

Molecular Plant-Microbe Interactions - Tập 32 Số 7 - Trang 876-887 - 2019
Tagginahalli N. Shivakumara1, Tushar K. Dutta1, Sonam Chaudhary1, Stephan H. von Reuß2, Valerie M. Williamson3
1Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
2Institute of Chemistry, University of Neuchâtel, Neuchâtel, Avenue de Bellevaux 51, Switzerland
3Department of Plant Pathology, University of California, Davis, CA 95616, U.S.A.

Tóm tắt

Nematode chemosensation is a vital component of their host-seeking behavior. The globally important phytonematode Meloidogyne incognita perceives and responds (via sensory organs such as amphids and phasmids) differentially to various chemical cues emanating from the rhizosphere during the course of host finding. However, compared with the free-living worm Caenorhabditis elegans, the molecular intricacies behind the plant nematode chemotaxis are a yet-unexploited territory. In the present study, four putative chemosensory genes of M. incognita, namely, Mi-odr-1, Mi-odr-3, Mi-tax-2, and Mi-tax-4 were molecularly characterized. Mi-odr-1 mRNA was found to be expressed in the cell bodies of amphidial neurons and phasmids of M. incognita. Mi-odr-1, Mi-odr-3, Mi-tax-2, and Mi-tax-4 transcripts were highly expressed in early life stages of M. incognita, consistent with a role of these genes in host recognition. Functional characterization of Mi-odr-1, Mi-odr-3, Mi-tax-2, and Mi-tax-4 via RNA interference revealed behavioral defects in M. incognita and perturbed attraction to host roots in Pluronic gel medium. Knockdown of Mi-odr-1, Mi-odr-3, Mi-tax-2, and Mi-tax-4 resulted in defective chemotaxis of M. incognita to various volatile compounds (alcohol, ketone, aromatic compound, ester, thiazole, pyrazine), nonvolatiles of plant origin (carbohydrate, phytohormone, organic acid, amino acid, phenolic), and host root exudates in an agar-Pluronic gel–based assay plate. In addition, ascaroside-mediated signaling was impeded by downregulation of chemosensory genes. This new information that behavioral response in M. incognita is modulated by specific olfactory genes can be extended to understand chemotaxis in other nematodes.

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Tài liệu tham khảo

10.1038/nbt.1482

10.1023/A:1022888900465

10.1016/j.ijpara.2008.05.003

10.1016/0092-8674(93)80053-H

Bargmann C. I., 1997, C. elegans, 717

10.1371/journal.ppat.1000192

10.1371/journal.pgen.1006777

Byrd D. W., 1983, J. Nematol., 15, 142

10.1038/s41598-018-29165-4

10.1016/j.cub.2012.03.024

10.1016/S0896-6273(00)80201-9

Coburn C. M., 1998, Development, 125, 249, 10.1242/dev.125.2.249

10.1016/0896-6273(95)90224-4

10.1051/parasite/2008153310

10.1016/j.ijpara.2009.05.006

10.1016/j.bbrc.2017.06.143

10.3389/fmicb.2014.00760

Dutta T. K., 2016, Int. J. Nematol., 46, 73

10.1163/156854111X612306

10.1163/138855410X528262

10.1094/PHYTO-01-13-0019-RVW

Fleming T. R., 2017, J. Nematol., 49, 462

10.1017/S0031182000059394

10.1016/S0960-9822(02)00813-8

10.1016/j.apsoil.2010.09.005

10.1111/mpp.12057

10.1146/annurev-phyto-080516-035434

10.1016/S0020-7519(02)00084-X

10.1016/S0896-6273(00)80202-0

10.1023/A:1022269821379

10.1016/j.gene.2017.03.042

10.1016/S0896-6273(00)81061-2

10.1534/genetics.103.024786

10.1017/S0031182011002071

10.1006/meth.2001.1262

10.1186/s13071-017-2353-8

10.1895/wormbook.1.155.1

10.1038/ncomms8795

10.1079/9781845934927.0001

10.1093/nar/gki419

10.3389/fpls.2017.01987

10.1146/annurev.phyto.34.1.181

Perry R. N., 2005, Russ. J. Nematol., 13, 83

10.1093/bioinformatics/14.9.817

10.1016/j.conb.2016.09.002

10.1098/rsif.2010.0417

10.1016/S0896-6273(00)80434-1

10.1146/annurev.phyto.112408.132605

10.1016/S0092-8674(00)81068-5

10.3389/fpls.2017.00473

10.21307/jofnem-2018-047

10.1163/15685411-00003003

10.1094/PHYTO.1997.87.8.839

10.1371/journal.pbio.1001237

10.1093/gbe/evx201

10.1371/journal.pone.0061259

10.1094/MPMI.2002.15.8.747

10.1007/978-94-011-5596-0_5

10.1163/156854109X447024

10.1371/journal.pone.0017475

10.1111/j.1744-7348.1965.tb07864.x

10.1094/MPMI.2001.14.10.1206

10.1111/j.1365-3059.2007.01607.x

10.1163/156854106776179953

10.1016/S0020-7519(01)00315-0

10.1073/pnas.94.7.3384

10.1038/nature04216

10.1094/PHYTO.2000.90.11.1239

10.1146/annurev.py.22.090184.000523

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Molecular Plant-Microbe Interactions

Tập 32 Số 7

876-887

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