Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation

Journal of Cell Biology - Tập 184 Số 2 - Trang 205-213 - 2009
Yanming Wang1, Ming Li1, Sonja C. Stadler2,3, Sarah Correll4, Pingxin Li1, Danchen Wang1, Ryo Hayama2, Lauriebeth Leonelli2, Hyunsil Han5,6,7, Sergei A. Grigoryev4, C. David Allis3, Scott A. Coonrod2
1Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802 1
2Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853 2
3Laboratory of Chromatin Biology, The Rockefeller University, New York, NY 10065 3
4Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033 4
5Department of Immunology, Weill Medical College of Cornell University, New York, NY 10021 5 and 6
6Department of Microbiology 5 and 6
7Department of Microbiology and Department of Immunology, Weill Medical College of Cornell University, New York, NY 10021

Tóm tắt

Peripheral blood neutrophils form highly decondensed chromatin structures, termed neutrophil extracellular traps (NETs), that have been implicated in innate immune response to bacterial infection. Neutrophils express high levels of peptidylarginine deiminase 4 (PAD4), which catalyzes histone citrullination. However, whether PAD4 or histone citrullination plays a role in chromatin structure in neutrophils is unclear. In this study, we show that the hypercitrullination of histones by PAD4 mediates chromatin decondensation. Histone hypercitrullination is detected on highly decondensed chromatin in HL-60 granulocytes and blood neutrophils. The inhibition of PAD4 decreases histone hypercitrullination and the formation of NET-like structures, whereas PAD4 treatment of HL-60 cells facilitates these processes. The loss of heterochromatin and multilobular nuclear structures is detected in HL-60 granulocytes after PAD4 activation. Importantly, citrullination of biochemically defined avian nucleosome arrays inhibits their compaction by the linker histone H5 to form higher order chromatin structures. Together, these results suggest that histone hypercitrullination has important functions in chromatin decondensation in granulocytes/neutrophils.

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Journal of Cell Biology

Tập 184 Số 2

205-213

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