Lucía Pérez-Álvarez1, Rocı́o Carmona1, Mercedes Muñoz1, Elena Delgado1, Michael M. Thomson1, Gerardo Contreras1, J. Pedreira2, Rafael Rodríguez Real3, Elena Vázquez de Parga1, Leandro Medrano1, José Antonio Franco Taboada4, Rafael Nájera1, A Agulla5, Ana Mariño5, Soledad López‐Calvo6, Pedreira Jd6, Antonio Aguilera7, Elena Losada7, Arturo Prieto7, Juan Corredoira8, MJ López- Alvarez8, A. Rodríguez8, Matilde Bustillo9, Isabel García-Bermejo9, Juan Carlos Piñeiro-Fernández9, Ronald Rodríguez10, Celia Miralles11, Antonio Ocampo11, R. Rodriguez-Real12, Julio Diz-Arén12, Rafael Ojea de Castro12, LE Morano13, R Pérez-Rodríguez13, José Manuel Torres13
1Area de Patogenia Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain
2Unidade de Seguimiento de VIH/SIDA, Hospital Juan Canalejo, La Coruña, Spain
3Complejo Hospitalario, Pontevedra, Spain
4Consellería de Sanidade e Servicios Sociais, Dirección Xeral de Saúde Pública, Xunta de Galicia, Santiago de Compostela, Spain
5Hospital Arquitecto Marcide, Ferrol (La Coruña)
6Hospital Juan Canalejo. La Coruña.
7Complejo Hospitalario Universitario de Santiago (La Coruña)
8Hospital Xeral-Calde (Lugo)
9Hospital Nuestra Señora del Cristal (Orense)
10Hospital Provincial Santa María Madre (Orense)
11Hospital Xeral-Cíes, Vigo (Pontevedra)
12Complejo Hospitalario de Pontevedra
13Hospital Meixoeiro, Vigo (Pontevedra).
Tóm tắt
The objectives of this study are to describe the incidence of non-B and recombinant HIV-1 strains in newly diagnosed HIV-1 infections in Galicia, northwest of Spain, during a 2-year period (May 2000 to June 2002), and the frequency of resistance-associated mutations in reverse transcriptase (RT) and protease (PR) genes, analysing the polymorphisms more frequently detected in non-B and recombinant viruses. All newly diagnosed HIV-1-infected patients attending the nine public hospitals of the seven main cities of Galicia were included in this study. RT, PR and V3 regions from HIV-1 RNA plasma were amplified and sequenced, being the corrected sequences sent to the Stanford HIV RT and Protease Sequence Database. Nineteen of 85 patients (22.3%) were infected by non-B or recombinant viruses: three subtype C, two G, one F1, one Dpol/A1V3, five CRF02_AG, one CRF14_BG, five BGpol/BV3and one UKpol/UV3(U, unknown fragment). Eleven of these 19 patients (57.9%) were foreign individuals living in Galicia infected through heterosexual contact, and the other eight (42.1%) were Spanish intravenous drug users who had shared injection equipment. Five of 85 patients (5.9%), all infected with B subtype viruses, showed resistance-associated mutations in RT (M184V, M41L, L210W, T215Y/D and K219Q). In one patient (1.2%) infected with a subtype G strain, resistance-associated mutations in PR (K20I+M36I+M46I+V82I) were detected. In subtype B viruses resistance mutations in PR were not detected. Several polymorphisms in RT: D123S, Q174K, D177E, T200A, V245Q, and PR: I13V, K20I, M36I, R41K, H69K, L89M were detected more frequently in non-B and recombinants than in B strains ( P<0.01 to P<0.001). This study reports a high incidence (22.3%) of newly diagnosed patients infected by different non-B and recombinant HIV-1 strains, in a geographical area of Spain, showing also a high frequency of polymorphisms in RT and PR genes.
