Heterozygosity and segregation in Candida albicans
Tóm tắt
Strain FC18 of the pathogenic yeast Candida albicans is heterozygous at a gene required for synthesis of methionine and cysteine, and yields auxotrophic derivatives by induced mitotic segregation. Derivatives of FC18 are heterozygous at other genes. Genetic analysis of C. albicans is feasible using induced mitotic recombination.
Tài liệu tham khảo
Bish JT, Sarachek A (1967) Influences of temperature and adenine concentration upon the cultural instability of a red adenine auxotroph of Candida albicans. Mycologia 59:671–688
Fink GR (1970) The biochemical genetics of yeast. Meth Enzymol 17A:59–78
Henson OE, McClary DO (1979) Growth inhibition of Candida albicans by folate pathway inhibitors. Their potential in the selection of auxotrophs. Antonie von Leeuwonhoek. J Microbiol Serol 45:211–223
Hesse G, Lindner R, Krebs D (1975) Schnelle fluorotometrische deoxyribosespezifische DNS-bestimmung an nicht desintegrierten mikroorganismen mit 3–5 diaminobenzoesäure (DABA). I. Chemisch-analytische Methodik und anwendung auf hefepopulationen. Z Allg Mikrobiol 15:9–18
Holliday R (1961) Induced mitotic crossing-over in Ustilago maydis. Genet Res Camb 2:231–248
James AP (1954) Relative frequencies of sectored and non-sectored mutant colonies in yeast as a function of ultraviolet dose. J Bacteriol 67:237–242
James AP (1955) A genetic analysis of sectoring in ultraviolet-induced variant colonies of yeast. Genetics 40:204–213
James AP, Lee-Whiting B (1955) Radiation-induced genetic segregation in vegetative cells of diploid yeast. Genetics 40:826–831
Kwon-Chung KH, Hill WB (1970) Studies on the pink, adenine deficient, strains of Candida albicans. I. Cultural and morphological characteristics. Sabouraudia 8:48–59
Nakai S, Mortimer RK (1969) Studies of the genetic mechanism of radiation induced mitotic segregation in yeast. Mol Gen Genet 103:329–338
Olaiya AF, Sogin SJ (1979) Ploidy determination of Candida albicans. J Bacteriol 140:1043–1049
Pontecorvo G, Roper JA, Forbes E (1953) Genetic recombination without sexual reproduction in Aspergillus niger. J Gen Microbiol 8:198–210
Pontecorvo G, Sermonti G (1954) Parasexual recombination in Penicillum chrysogenum. J Gen Microbiol 11:94–104
Sarachek A (1964) Promotion or retardation of the growth of adenine auxotrophs in Candida albicans by purines, pyrimidines, and nucleosides. Antonie von Leeuwonhoek. J Microbiol Serol 30:289–302
Stern C (1936) Somatic crossing over and segregation in Drosophila melanogaster. Genetics 21:675–730
Williamson DH, Fennell DJ (1975) The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA. In: Methods in cell biology, XII ed Prescott. Academic Press, New York, p 335