Hepatotoxicity and cholestasis in rats induced by the sesquiterpene, 9‐oxo‐10,11‐dehydroageraphorone, isolated from Eupatorium adenophorum

Journal of Biochemical and Molecular Toxicology - Tập 15 Số 5 - Trang 279-286 - 2001
Renu Bhardwaj1, Ajay Pratap Singh1, Om P. Sharma2, Rajinder Dawra2, N. P. Kurade2, Shashi B. Mahato3
1Department of Chemistry and Biochemistry, Himachal Pradesh Krishi Vishvavidyalaya, Palampur 176 062, HP, India
2Biochemistry Laboratory, Indian Veterinary Research Institute, Regional Station, Palampur 176 061, HP, India
3Indian Institute of Chemical Biology, Jadavpur, Calcutta 700 032, India

Tóm tắt

Abstract

Eupatorium adenophorum leaves cause hepatotoxicity and cholestasis in rats. The hepatotoxicant has been characterized as 9‐oxo‐10,11‐dehydroageraphorone (ODA), a cadinene sesquiterpene. Oral administration of ODA, mixed in feed to rats, caused jaundice in 24 h. The liver of the intoxicated animals had focal areas of hepatocellular necrosis, proliferation, and dilation of bile ducts with degenerative changes in the lining epithelium. There was marked increase in the conjugated form of plasma bilirubin and in the activities of the enzymes glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, lactate dehydrogenase, γ‐glutamyltranspeptidase, glutamate dehydrogenase, and 5′‐nucleotidase. The histopathological lesions in liver and biochemical profile of marker enzymes show that ODA induced hepatotoxicity and cholestasis in rats. This is the first report on the toxicity of a cadinene sesquiterpene in rats. © 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:279–286, 2001

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Journal of Biochemical and Molecular Toxicology

Tập 15 Số 5

279-286

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