Hepatopancreatic multi‐transcript expression patterns in the crayfish Cherax quadricarinatus during the moult cycle

Insect Molecular Biology - Tập 16 Số 6 - Trang 661-674 - 2007
Yana Yudkovski1, Assaf Shechter2,3, Vered Chalifa‐Caspi3, Meirav Auslander4,1, Ron Ophir5, Chantal Dauphin‐Villemant6, Robert Robbins7, Amir Sagi2,3, Moshe Tom1
1Israel Oceanographic and Limnological Research, Haifa, Israel
2Department of Life Sciences and the
3National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer Sheva, Israel
4Faculty of Civil and Environmental Engineering, Technion-Israel Institute of Technology, Technion City, Haifa, Israel
5Weizmann Institute of Science, Rehovot, Israel
6FRE2852 CNRS-UPMC, Laboratoire Protéines Biochimie structurale et fonctionnelle, Paris, France
7Vanderbilt University School of Medicine, Nashville, TN USA

Tóm tắt

AbstractAlterations of hepatopancreatic multi‐transcript expression patterns, related to induced moult cycle, were identified in male Cherax quadricarinatus through cDNA microarray hybridizations of hepatopancreatic transcript populations. Moult was induced by X–organ sinus gland extirpation or by repeated injections of 20‐hydroxyecdysone. Manipulated males were sacrificed at premoult or early postmoult, and a reference population was sacrificed at intermoult. Differentially expressed genes among the four combinations of two induction methods and two moult stages were identified. Biologically interesting clusters revealing concurrently changing transcript expressions across treatments were selected, characterized by a general shift of expression throughout premoult and early postmoult vs. intermoult, or by different premoult vs. postmoult expressions. A number of genes were differentially expressed in 20‐hydroxyecdysone‐injected crayfish vs. X–organ sinus gland extirpated males.

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Insect Molecular Biology

Tập 16 Số 6

661-674

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