Hematotoxicological analysis of surface‐modified and ‐unmodified chitosan nanoparticles

Journal of Biomedical Materials Research - Part A - Tập 101 Số 10 - Trang 2957-2966 - 2013
Ragima Nadesh1,2, Dhanya Narayanan1,2, Sreerekha P.R.1, Sajini Vadakumpully1, Ullas Mony1, Manzoor Koyakkutty1, Shantikumar V. Nair1, Deepthy Menon1
1Amrita Centre for Nanosciences & Molecular Medicine Amrita Institute of Medical Sciences & Research Centre Amrita Vishwa Vidyapeetham Kochi 682041 Kerala India
2these authors contributed equally to this work

Tóm tắt

The increasing interest in using chitosan nanoparticles for controlled drug delivery is hampered by its blood incompatibility, especially for intravenous applications. This study investigated the effects of processing solvents (acetic acid/lactic acid), dispersing media (acidic medium/saline), and surface modifiers (polyethylene glycol, polyvinyl alcohol, and ethylenediaminetetraacetatic acid) on the hemocompatibility of chitosan. Blood compatibility of chitosan nanoparticles prepared by ionotropic gelation with altered surface chemistry was evaluated by assessing their hemolytic activity, platelet aggregation, coagulation, and cytokine induction. It was observed that nanoparticles prepared in lactic acid and dispersed in saline did not show hemolysis, platelet aggregation, or coagulation, whereas nanoparticles prepared in acetic acid showed strong hemolysis. Surface modifiers were not observed to significantly affect blood compatibility, with the exception of EDTA, which delayed blood clotting times. Thus, chitosan nanoparticles prepared in lactic acid and dispersed in saline may be an ideal nanocarrier for parenteral applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:2957–2966, 2013.

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