Hedgehog signaling patterns the outgrowth of unpaired skeletal appendages in zebrafish

BMC Developmental Biology - 2007
Yavor Hadzhiev1, Zsolt Lele2, Simone Schindler1, Stephen W Wilson3, Per Ahlberg4, Uwe Strähle1, Ferenc Müller1
1Institute of Toxicology and Genetics, Karlsruhe, Germany
2Department of Gene Technology and Developmental Neurobiology, Institute for Experimental Medicine, Budapest, Hungary
3Department of Anatomy and Developmental Biology, University College London, London, UK
4Department of Evolutionary Organismal Biology, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden

Tóm tắt

Little is known about the control of the development of vertebrate unpaired appendages such as the caudal fin, one of the key morphological specializations of fishes. Recent analysis of lamprey and dogshark median fins suggests the co-option of some molecular mechanisms between paired and median in Chondrichthyes. However, the extent to which the molecular mechanisms patterning paired and median fins are shared remains unknown. Here we provide molecular description of the initial ontogeny of the median fins in zebrafish and present several independent lines of evidence that Sonic hedgehog signaling emanating from the embryonic midline is essential for establishment and outgrowth of the caudal fin primordium. However, gene expression analysis shows that the primordium of the adult caudal fin does not harbor a Sonic hedgehog-expressing domain equivalent to the Shh secreting zone of polarizing activity (ZPA) of paired appendages. Our results suggest that Hedgehog proteins can regulate skeletal appendage outgrowth independent of a ZPA and demonstrates an unexpected mechanism for mediating Shh signals in a median fin primordium. The median fins evolved before paired fins in early craniates, thus the patterning of the median fins may be an ancestral mechanism that controls the outgrowth of skeletogenic appendages in vertebrates.

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