HPLC method for simultaneous determination of clobazam and N‐desmethylclobazam in human serum, rat serum and rat brain homogenates

Biomedical Chromatography - Tập 3 Số 2 - Trang 79-81 - 1989
Wieslaw R. Gaźik1, Jerzy Podleśany1, Marian Filipek1
1Laboratory Diagnostic Department, Memorial Hospital, Child Health Centre, Al. Dzieci Polskich 20, 04‐736 Warsaw, Poland

Tóm tắt

Abstract

A method for simultaneous determination of clobazam (CBZ) and its active metabolite N‐desmethylclobazam (DCBZ) in various biological samples by RP‐HPLC with UV detection is described. The determination of both CBZ and DCBZ is performed without derivatization. The internal standard is diazepam. The method is rapid and simple with sensitivity limits of 10 ng/mL for both CBZ and DCBZ and is suitable for routine analysis as well as for animal studies.

Từ khóa


Tài liệu tham khảo

10.2165/00003495-198020030-00001

Cano J. P., 1981, Roy. Soc. Med. (Internat. Congress and Symposium Series), 43, 169

10.1111/j.1365-2125.1979.tb05920.x

Gastaut H., 1978, Nouv. Press Med., 7, 2400

10.1111/j.1528-1157.1979.tb04825.x

Hajdú P., 1980, J. Clin. Chem. Clin. Biochem., 18, 109

10.1111/j.1365-2125.1979.tb04663.x

10.1111/j.1365-2125.1984.tb02558.x

10.1111/j.1365-2125.1979.tb04684.x

Levy R. H., 1983, Drug. Met. Dispos., 11, 286

Martin A. A., 1981, Roy Soc. Med. (Internat. Congress and Symposium Series)., 43, 151

Peña M. I. A., 1986, J. Clin. Chem. Clin. Biochem., 24, 647

10.1039/an9840900813

10.1111/j.1365-2125.1979.tb04665.x

10.1001/archneur.1986.00520080064023

10.1016/S0378-4347(00)80749-5

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Thông tin xuất bản

Biomedical Chromatography

Tập 3 Số 2

79-81

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