HPLC‐ELSD determination of kanamycin B in the presence of kanamycin A in fermentation broth

Biomedical Chromatography - Tập 29 Số 3 - Trang 396-401 - 2015
Yong Zhang1, Hui‐Min He1, Jin Zhang1, F Liu1, Chao Li1, Bing‐Wu Wang1, Renzhong Qiao1,2
1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China
2State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, People's Republic of China

Tóm tắt

AbstractA novel method for the direct determination of kanamycin B in the presence of kanamycin A in fermentation broth using high performance liquid chromatography with evaporative light scattering detector (HPLC‐ELSD) was developed. An Agilent Technologies C18 column was utilized, evaporation temperature of 40°C and nitrogen pressure of 3.5 bar, the optimized mobile phase was water–acetonitrile (65:35, v/v), containing 11.6 mm heptafluorobutyric acid (isocratic elution with flow rate of 0.5 mL/min) with the gain 11. Kanamycin B was eluted at 5.6 min with an asymmetry factor of 1.827. The method showed good linearity over the concentration range of 0.05 to 0.80 mg/mL for the kanamycin B (r2 = 0.9987). The intra‐day and inter‐day coefficients of variation obtained from kanamycin B were less than 4.3%. Mean recovery of kanamycin B from spiked fermentation broth was 95%. The developed method was applied to the determination of kanamycin B without any interference from other constituents in the fermentation broth. This method offers simple, rapid and quantitative detection of kanamycin B. Copyright © 2014 John Wiley & Sons, Ltd.

Từ khóa


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Thông tin xuất bản

Biomedical Chromatography

Tập 29 Số 3

396-401

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