Gut bacteria–host metabolic interplay during conventionalisation of the mouse germfree colon

ISME Journal - Tập 7 Số 4 - Trang 743-755 - 2013
Sahar El Aidy1,2, Muriel Derrien1,2, Claire A Merrifield3, Florence Levenez4, Joël Doré4, Mark V. Boekschoten5, Jan P. Dekker6,2, Elaine Holmes3, Erwin G. Zoetendal1,1, Peter van Baarlen7, Sandrine P. Claus8, Michiel Kleerebezem9,7,1,1
1Laboratory of Microbiology, Wageningen University, Dreijenplein , Wageningen, The Netherlands
2Top Institute Food and Nutrition , Wageningen, Wageningen, The Netherlands
3Biomolecular Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, UK
4INRA, UMR1319 , F-78350, Jouy-en-Josas, France
5Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands
6Department of Animal Sciences, Wageningen University, Wageningen, The Netherlands
7Host-Microbe Interactomics, Wageningen University, Wageningen, The Netherlands
8Department of Food and Nutritional Sciences, The University of Reading, Reading, UK
9Health Department, NIZO food research , Ede, The Netherlands

Tóm tắt

Abstract The interplay between dietary nutrients, gut microbiota and mammalian host tissues of the gastrointestinal tract is recognised as highly relevant for host health. Combined transcriptome, metabonome and microbial profiling tools were employed to analyse the dynamic responses of germfree mouse colonic mucosa to colonisation by normal mouse microbiota (conventionalisation) at different time-points during 16 days. The colonising microbiota showed a shift from early (days 1 and 2) to later colonisers (days 8 and 16). The dynamic changes in the microbial community were rapidly reflected by the urine metabolic profiles (day 1) and at later stages (day 4 onward) by the colon mucosa transcriptome and metabolic profiles. Correlations of host transcriptomes, metabolite patterns and microbiota composition revealed associations between Bacilli and Proteobacteria, and differential expression of host genes involved in energy and anabolic metabolism. Differential gene expression correlated with scyllo- and myo-inositol, glutamine, glycine and alanine levels in colonic tissues during the time span of conventionalisation. Our combined time-resolved analyses may help to expand the understanding of host–microbe molecular interactions during the microbial establishment.

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ISME Journal

Tập 7 Số 4

743-755

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