Graphene Nanosheets to Improve Physico-Mechanical Properties of Bioactive Calcium Silicate Cements

Materials - Tập 10 Số 6 - Trang 606
Nileshkumar Dubey1, Sneha Sundar Rajan1, Yuri Dal Bello2, Kyung‐San Min3, Vinícius Rosa4,1
1Faculty of Dentistry, National University of Singapore, Singapore 119083, Singapore
2Faculty of Dentistry, University of Passo Fundo, Passo Fundo 99052-900, Brazil;
3School of Dentistry and Institute of Oral Bioscience, Chonbuk National University, Jeonju-si 54896, Korea;
4Centre for Advanced 2D Materials and Graphene Research Centre, National University of Singapore, Singapore, 117546, Singapore

Tóm tắt

Bioactive calcium silicate cements are widely used to induce mineralization, to cement prosthetic parts, in the management of tooth perforations, and other areas. Nonetheless, they can present clinical disadvantages, such as long setting time and modest physico-mechanical properties. The objective of this work was to evaluate the potential of graphene nanosheets (GNS) to improve two bioactive cements. GNS were obtained via reduction of graphite oxide. GNS were mixed (1, 3, 5, and 7 wt %) with Biodentine (BIO) and Endocem Zr (ECZ), and the effects on setting time, hardness, push-out strength, pH profile, cell proliferation, and mineralization were evaluated. Statistics were performed with two-way ANOVA and Tukey test (α = 0.05). GNS has not interfered in the composition of the set cements as confirmed by Raman, FT-IR and XRD. GNS (1 and 3 wt %) shortened the setting time, increased hardness of both materials but decreased significantly the push-out strength of ECZ. pH was not affected but 1 wt % and 7 wt % to ECZ and 5 wt % to BIO increased the mineralization compared to the controls. In summary, GNS may be an alternative to improve the physico-mechanical properties and bioactivity of cements. Nonetheless, the use of GNS may not be advised for all materials when effective bonding is a concern.

Từ khóa


Tài liệu tham khảo

Caron, 2014, Use of a new retrograde filling material (biodentine) for endodontic surgery: Two case reports, Int. J. Oral Sci., 6, 250, 10.1038/ijos.2014.25

Vidal, 2016, Apical closure in apexification: A review and case report of apexification treatment of an immature permanent tooth with biodentine, J. Endod., 42, 730, 10.1016/j.joen.2016.02.007

Michel, 2016, In vitro evaluation of different dental materials used for the treatment of extensive cervical root defects using human periodontal cells, Clin. Oral Investig., 21, 753, 10.1007/s00784-016-1830-3

Marconyak, 2016, A comparison of coronal tooth discoloration elicited by various endodontic reparative materials, J. Endod., 42, 470, 10.1016/j.joen.2015.10.013

Kang, 2015, Color changes of teeth after treatment with various mineral trioxide aggregate–based materials: An ex vivo study, J. Endod., 41, 737, 10.1016/j.joen.2015.01.019

Hakki, 2009, Effects of mineral trioxide aggregate on cell survival, gene expression associated with mineralized tissues, and biomineralization of cementoblasts, J. Endod., 35, 513, 10.1016/j.joen.2008.12.016

Min, 2009, The combined effect of mineral trioxide aggregate and enamel matrix derivative on odontoblastic differentiation in human dental pulp cells, J. Endod., 35, 847, 10.1016/j.joen.2009.03.014

Maeda, 2010, Mineral trioxide aggregate induces bone morphogenetic protein-2 expression and calcification in human periodontal ligament cells, J. Endod., 36, 647, 10.1016/j.joen.2009.12.024

Natu, 2015, Bioactivity, physical and chemical properties of mta mixed with propylene glycol, Int. J. Oral Sci., 23, 405

Kaup, 2015, An in vitro study of different material properties of biodentine compared to proroot mta, Head Face Med., 11, 16, 10.1186/s13005-015-0074-9

Qing, 2007, Influence of nano-sio2 addition on properties of hardened cement paste as compared with silica fume, Constr. Build. Mater., 21, 539, 10.1016/j.conbuildmat.2005.09.001

Kong, Y.-M., Kim, S., Kim, H.-E., and Lee, I.-S. (2010). Reinforcement of hydroxyapatite bioceramic by addition of zro2 coated with al2o3. ChemInform, 31.

Makar, J., Margeson, J., and Luh, J. (2005, January 22–24). Carbon nanotube/cement composites-early results and potential application. Proceedings of the 3rd International Conference on Construction Materials: Performance, Innovations and Structural Implications, Vancouver, BC, Canada.

Musso, 2009, Influence of carbon nanotubes structure on the mechanical behavior of cement composites, Compos. Sci. Technol., 69, 1985, 10.1016/j.compscitech.2009.05.002

Bittnar, Z., Bartos, P.J.M., Němeček, J., Šmilauer, V., and Zeman, J. (2009). Mortar and concrete reinforced with nanomaterials. Nanotechnology in Construction 3, Springer. [1st ed.].

Morsy, 2011, Hybrid effect of carbon nanotube and nano-clay on physico-mechanical properties of cement mortar, Constr. Build. Mater., 25, 145, 10.1016/j.conbuildmat.2010.06.046

Dubey, 2015, Graphene: A versatile carbon-based material for bone tissue engineering, Stem Cells Int., 2015, 804213, 10.1155/2015/804213

Xie, 2015, Two and three-dimensional graphene substrates to magnify osteogenic differentiation of periodontal ligament stem cells, Carbon, 93, 266, 10.1016/j.carbon.2015.05.071

Rosa, 2016, Graphene oxide-based substrate: Physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cells, Dent. Mater., 32, 1019, 10.1016/j.dental.2016.05.008

Pan, Z., Duan, W., Li, D., and Collins, F. (2013). Graphene Oxide Reinforced Cement and Concrete. (WO2013096990 A1), Patent.

Gong, K., Pan, Z., Korayem, A.H., Qiu, L., Li, D., Collins, F., Wang, C.M., and Duan, W.H. (2015). Reinforcing effects of graphene oxide on portland cement paste. J. Mater. Civ. Eng., 27.

Lv, 2014, Use of graphene oxide nanosheets to regulate the microstructure of hardened cement paste to increase its strength and toughness, CrystEngComm, 16, 8508, 10.1039/C4CE00684D

Zhang, 2013, A tough graphene nanosheet/hydroxyapatite composite with improved in vitro biocompatibility, Carbon, 61, 105, 10.1016/j.carbon.2013.04.074

Mehrali, M., Moghaddam, E., Shirazi, S.F.S., Baradaran, S., Mehrali, M., Latibari, S.T., Metselaar, H.S.C., Kadri, N.A., Zandi, K., and Osman, N.A.A. (2014). Mechanical and in vitro biological performance of graphene nanoplatelets reinforced calcium silicate composite. PLoS ONE, 9.

Thema, 2013, Synthesis and characterization of graphene thin films by chemical reduction of exfoliated and intercalated graphite oxide, J. Chem., 2013, 150536, 10.1155/2013/150536

Marciano, 2015, Dental discoloration caused by bismuth oxide in mta in the presence of sodium hypochlorite, Clin. Oral Investig., 19, 2201, 10.1007/s00784-015-1466-8

Sarkar, 2005, Physicochemical basis of the biologic properties of mineral trioxide aggregate, J. Endod., 31, 97, 10.1097/01.DON.0000133155.04468.41

Grech, 2013, Characterization of set intermediate restorative material, biodentine, bioaggregate and a prototype calcium silicate cement for use as root-end filling materials, Int. Endod. J., 46, 632, 10.1111/iej.12039

Jensen, 2010, Clinical results with two different methods of root-end preparation and filling in apical surgery: Mineral trioxide aggregate and adhesive resin composite, J. Endod., 36, 1122, 10.1016/j.joen.2010.03.040

Chong, 2003, A prospective clinical study of mineral trioxide aggregate and irm when used as root-end filling materials in endodontic surgery, Int. Endod. J., 36, 520, 10.1046/j.1365-2591.2003.00682.x

Choi, 2013, Biological effects and washout resistance of a newly developed fast-setting pozzolan cement, J. Endod., 39, 467, 10.1016/j.joen.2012.11.023

Camilleri, 2013, Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, biodentine and mta angelus, Dent. Mater., 29, 580, 10.1016/j.dental.2013.03.007

Manzur, 2016, Potential of carbon nanotube reinforced cement composites as concrete repair material, J. Nanomater., 2016, 1421959, 10.1155/2016/1421959

Manzur, 2014, Optimum mix ratio for carbon nanotubes in cement mortar, KSCE Civ. Eng., 19, 1405, 10.1007/s12205-014-0721-x

Siddique, 2014, Effect of carbon nanotubes on properties of cement mortars, Constr. Build. Mater., 50, 116, 10.1016/j.conbuildmat.2013.09.019

Ahn, 2005, The effect of zirconia reinforcing agents on the microstructure and mechanical properties of hydroxyapatite-based nanocomposites, J. Am. Ceram. Soc., 88, 3374, 10.1111/j.1551-2916.2005.00636.x

Aggarwal, 2013, Comparative evaluation of push-out bond strength of proroot mta, biodentine, and mta plus in furcation perforation repair, J. Conserv. Dent., 16, 462, 10.4103/0972-0707.117504

Guneser, 2013, Effect of various endodontic irrigants on the push-out bond strength of biodentine and conventional root perforation repair materials, J. Endod., 39, 380, 10.1016/j.joen.2012.11.033

Han, 2011, Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine, Int. Endod. J., 44, 1081, 10.1111/j.1365-2591.2011.01924.x

Nikhil, 2014, Effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine, J. Conserv. Dent., 17, 271, 10.4103/0972-0707.131795

Bhavana, 2015, Evaluation of antibacterial and antifungal activity of new calcium-based cement (biodentine) compared to mta and glass ionomer cement, J. Conserv. Dent., 18, 44, 10.4103/0972-0707.148892

Wataha, 2012, Predicting clinical biological responses to dental materials, Dent. Mater., 28, 23, 10.1016/j.dental.2011.08.595

Wei, 2011, Synthesis of high-performance graphene nanosheets by thermal reduction of graphene oxide, Mater. Res. Bull., 46, 2131, 10.1016/j.materresbull.2011.06.036

(2013). Standard Test Method for Time of Setting of Hydraulic-Cement Paste by Gillmore Needles, American Association State Highway and Transportation Officials Standard. C266-13.

Valandro, 2007, Effect of mechanical cycling on the push-out bond strength of fiber posts adhesively bonded to human root dentin, Oper. Dent., 32, 579, 10.2341/06-165

Rosa, 2013, Dental pulp tissue engineering in full-length human root canals, J. Dent. Res., 92, 970, 10.1177/0022034513505772

Thông tin
Thông tin xuất bản

Materials

Tập 10 Số 6

606

Thông tin tác giả