Glycine stimulates striatal dopamine release in conscious rats

British Journal of Pharmacology - Tập 110 Số 1 - Trang 50-53 - 1993
Gal Yadid1, Karel Pacák1, Eliahu Golomb1, Judith Harvey−White1, Daniel Lieberman2, Irwin J. Kopin1, David S. Goldstein1
1Clinical Neuroscience Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, U.S.A.
2Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, U.S.A.

Tóm tắt

Glycine is an inhibitory neurotransmitter in the spinal cord and brainstem. The mechanism of this inhibition is via binding of glycine to specific receptors, increasing transmembrane Cl− conductance and hyperpolarizing neurones. Strychnine selectively antagonizes these effects. The role of glycinergic neurones in supraspinal regions is poorly understood. Effects of glycine on release of catecholamines in the striatum were examined by microdialysis in freely‐moving rats. Transcription of the genes encoding strychnine‐sensitive glycine receptors was assessed in the striatum and substantia nigra, by use of reverse transcription followed by the polymerase chain reaction. Glycine administered via the microdialysis probe dose‐dependently increased concentrations of dopamine and its metabolites, dihydroxyphenylacetic acid and homovanillic acid, in the perfusate, indicating increased local release and metabolism of dopamine. Strychnine markedly attenuated these responses. Whereas striatal tissue did not contain mRNA for either the adult or neonatal form of strychnine‐sensitive glycine receptor, nigral tissue contained a message for the adult form. The results suggest that dopaminergic cells in the substantia nigra synthesize strychnine‐sensitive glycine receptors and transport the receptors to terminals in the striatum. Occupation of the glycine receptors then exerts a net stimulatory effect on striatal dopamine release in vivo.

Từ khóa


Tài liệu tham khảo

BORMANN J., 1988, Chloride Channels and Their Modulation by Neurotransmitters and Drugs, 47

10.1016/0003-2697(87)90021-2

10.1007/BF00235443

10.1016/0006-8993(78)90064-1

10.1038/328215a0

10.1111/j.1471-4159.1982.tb08665.x

10.1016/0006-2952(79)90203-X

KRNJEVIK A., 1974, Chemical nature of synaptic transmission in vertebrates, Physiol. Rev., 54, 418, 10.1152/physrev.1974.54.2.418

10.1016/0896-6273(90)90346-H

10.1016/0006-8993(84)91316-7

10.1002/j.1460-2075.1991.tb07779.x

10.1002/syn.890050304

10.1016/0006-8993(92)91165-B

PAXINOS G., 1986, The Rat in Stereotaxic Coordinates

RAITERI M., 1990, Glycine stimulates [3H]‐noradrenaline release by activation of a strychnine‐sensitive receptor in rat hippocampus, Br. J. Pharmacol., 184, 239

10.1111/j.1471-4159.1979.tb11096.x

10.1111/j.1471-4159.1990.tb04913.x

10.1111/j.1476-5381.1983.tb10018.x

10.1007/BF00182730

10.1016/0014-2999(90)90579-U

10.1111/j.1476-5381.1991.tb12501.x

10.1016/0014-2999(92)90729-N

10.1073/pnas.70.10.2832

Thông tin
Thông tin xuất bản

British Journal of Pharmacology

Tập 110 Số 1

50-53

Thông tin tác giả