Glutathione Transferases and Glutathionylated Hemoglobin in Workers Exposed to Low Doses of 1,3-Butadiene

Cancer Epidemiology Biomarkers and Prevention - Tập 17 Số 11 - Trang 3004-3012 - 2008
Alessandra Primavera1, Silvia Fustinoni2, Antonino Biroccio3, Sabrina Ballerini4, Andrea Urbani5,6, Sergio Bernardini4, Giorgio Federici3,4, Enrico Capucci1, Maurizio Manno7, Mario Lo Bello1
11Dipartimento di Biologia, Università di Roma Tor Vergata, Via della Ricerca Scientifica snc;
22Dipartimento di Medicina del Lavoro e Igiene industriale, Università di Milano e Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy;
33Laboratorio di Chimica delle Proteine, Ospedale Pediatrico “Bambino Gesù”;
44Laboratorio di Biochimica Clinica, Dipartimento di Medicina di Laboratorio, Policlinico di Tor Vergata; and
55IRCCS “S.Lucia” CERCI, Via del Fosso di Fiorano snc, Rome, Italy;
66Dipartimento di Scienze Biomediche, Università “G. D'Annunzio” di Chieti e Pescara, Chieti-Pescara, Italy; and
77Sezione di Medicina del Lavoro e Tossicologia Occupazionale, Dipartimento di Scienze Mediche Preventive, Università di Napoli “Federico II,” Naples, Italy

Tóm tắt

Abstract We evaluated glutathione transferase (GST) activities and the levels of glutathionylated hemoglobin in the RBC of 42 workers exposed to 1,3-butadiene in a petrochemical plant, using 43 workers not exposed to 1,3-butadiene and 82 foresters as internal and external controls, respectively. Median 1,3-butadiene exposure levels were 1.5, 0.4, and 0.1 μg/m3 in 1,3-butadiene-exposed workers, in workers not directly exposed to 1,3-butadiene, and in foresters, respectively. In addition, we determined in the peripheral blood lymphocytes of the same individuals the presence of GST polymorphic genes GSTT1 and GSTM1 and the distribution of GSTP1 allelic variants. Comparing the mean values observed in petrochemical workers with those of control foresters, we found a marked decrease of GST enzymatic activity and a significant increase of glutathionylated hemoglobin in the petrochemical workers. A weak but significant negative correlation was found between levels of 1,3-butadiene exposure and GST activity, whereas a positive correlation was found between 1,3-butadiene exposure and glutathionylated hemoglobin. A negative correlation was also observed between GST activity and glutathionylated hemoglobin. No influence of confounders was observed. Using a multiple linear regression model, up to 50.6% and 41.9% of the variability observed in glutathionylated hemoglobin and GST activity, respectively, were explained by 1,3-butadiene exposure, working setting, and GSTT1 genotype. These results indicate that occupational exposure to 1,3-butadiene induces an oxidative stress that impairs the GST balance in RBC, and suggest that GST activity and glutathionylated hemoglobin could be recommended as promising biomarkers of effect in petrochemical workers. (Cancer Epidemiol Biomarkers Prev 2008;17(11):3004–12)

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Cancer Epidemiology Biomarkers and Prevention

Tập 17 Số 11

3004-3012

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