Glucose stimulates the biosynthesis of rat I and II insulin to an equal extent in isolated pancreatic islets

FEBS Letters - Tập 215 - Trang 179-182 - 1987
Christopher J. Rhodes1, Christine A. Lucas1, Philippe A. Halban1
1E.P. Joslin Research Laboratory, Joslin Diabetes Center, Brigham and Women's Hospital and Harvard Medical School, 1 Joslin Place, Boston, MA 02215, USA

Tóm tắt

The effects of glucose on insulin biosynthesis were studied by measuring the incorporation of radiolabelled amino acids into proinsulin/insulin in isolated rat islets. The islets were pulse labelled for 15 min with [3H]leucine (present in rat insulin I and II) or [35S]methionine (unique to rat insulin II) and then incubated for a 165 min post‐label (chase) period during which the majority of labelled proinsulin was converted to insulin but under conditions whereby > 95% of radiolabelled proinsulin or insulin was retained in the islets. The newly synthesized, labelled, insulin was analyzed by high performance liquid chromatography. Rat I and II insulin biosynthesis was stimulated by 16.7 mM glucose to the same extent.

Tài liệu tham khảo

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FEBS Letters

Tập 215

179-182

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